ArticleScientific reports2016
Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Kiss and spit metabolomics highlight the role of host purine metabolism during pathogen infection.mSphere · 2026Article
- Metabolic plasticity, essentiality and therapeutic potential of ribose-5-phosphate synthesis in Toxoplasma gondii.Nature communications · 2024Article
- Amino acid profiling to predict prognosis in patients with heart failure: an expert review.ESC heart failure · 2023Review
- Untargeted Metabolomics Uncovers the Essential Lysine Transporter inMetabolites · 2021Article
- Amino Acid Metabolism in Apicomplexan Parasites.Metabolites · 2021Review
- Structural, Functional, and Metabolic Alterations in Human Cerebrovascular Endothelial Cells duringMicroorganisms · 2020Article
- Raman-deuterium isotope probing to study metabolic activities of single bacterial cells in human intestinal microbiota.Microbial biotechnology · 2020Article
- Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.Journal of biophotonics · 2020Article
- Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis.Microorganisms · 2019Article
- Metabolic interactions betweenF1000Research · 2018Review
- Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis.Frontiers in cellular and infection microbiology · 2018Article
- Label-Free Raman Hyperspectral Imaging of Single Cells Cultured on Polymer Substrates.Applied spectroscopy · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
This study investigates the temporal and spatial interchange of the aromatic amino acid phenylalanine (Phe) between human retinal pigment epithelial cell line (ARPE-19) and tachyzoites of the apicomplexan protozoan parasite Toxoplasma gondii (T. gondii). Stable isotope labelling by amino acids in cell culture (SILAC) is combined with Raman micro-spectroscopy to selectively monitor the incorporation of deuterium-labelled Phe into proteins in individual live tachyzoites. Our results show a very rapid uptake of l-Phe(D8) by the intracellular growing parasite. T. gondii tachyzoites are capable of extracting l-Phe(D8) from host cells as soon as it invades the cell. l-Phe(D8) from the host cell completely replaces the l-Phe within T. gondii tachyzoites 7-9 hours after infection. A quantitative model based on Raman spectra allowed an estimation of the exchange rate of Phe as 0.5-1.6 × 10(4) molecules/s. On the other hand, extracellular tachyzoites were not able to consume l-Phe(D8) after 24 hours of infection. These findings further our understanding of the amino acid trafficking between host cells and this strictly intracellular parasite. In particular, this study highlights new aspects of the metabolism of amino acid Phe operative during the interaction between T. gondii and its host cell.
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