ArticleAntimicrobial agents and chemotherapy2016
Association of Novel Nonsynonymous Single Nucleotide Polymorphisms in ampD with Cephalosporin Resistance and Phylogenetic Variations in ampC, ampR, ompF, and ompC in Enterobacter cloacae Isolates That Are Highly Resistant to Carbapenems.
Article in Antimicrobial agents and chemotherapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 60 citations in OpenAlex.
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- Activity of cefepime, carbapenems and new β-lactam/β-lactamase inhibitor combinations onJAC-antimicrobial resistance · 2024Article
- Filling knowledge gaps related to AmpC-dependent β-lactam resistance in Enterobacter cloacae.Scientific reports · 2024Article
- Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria.Microorganisms · 2023Review
- Exploring clonality and virulence gene associations in bloodstream infections using whole-genome sequencing and clinical data.Frontiers in cellular and infection microbiology · 2023Article
- Antimicrobial Resistance and Its Drivers-A Review.Antibiotics (Basel, Switzerland) · 2022Review
- Class C β-Lactamases: Molecular Characteristics.Clinical microbiology reviews · 2022Review
- Combination of Whole Genome Sequencing and Metagenomics for Microbiological Diagnostics.International journal of molecular sciences · 2022Review
- Antimicrobial Resistance in Enterobacterales Recovered from Urinary Tract Infections in France.Pathogens (Basel, Switzerland) · 2022Article
- AmpC hyperproduction in a Cedecea davisae implant-associated bone infection during treatment: a case report and therapeutic implications.BMC infectious diseases · 2022Article
- Antimicrobial resistance and antibiotic consumption in intensive care units, Switzerland, 2009 to 2018.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2021Article
- Wide Distribution and Specific Resistance Pattern to Third-Generation Cephalosporins ofFrontiers in microbiology · 2021Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
InEnterobacter cloacae, the genetic lesions associated with derepression of the AmpC β-lactamase include diverse single nucleotide polymorphisms (SNPs) and/or indels in theampDandampRgenes and SNPs inampC, while diverse SNPs in the promoter region or SNPs/indels within the coding sequence of outer membrane proteins have been described to alter porin production leading to carbapenem resistance. We sought to define the underlying mechanisms conferring cephalosporin and carbapenem resistance in a collection ofE. cloacaeisolates with unusually high carbapenem resistance and no known carbapenemase and, in contrast to many previous studies, considered the SNPs we detected in relation to the multilocus sequence type (MLST)-based phylogeny of our collection. Whole-genome sequencing was applied on the most resistant isolates to seek novel carbapenemases, expression ofampCwas measured by reverse transcriptase PCR, and porin translation was detected by SDS-PAGE. SNPs occurring inampC,ampR,ompF, andompCgenes (and their promoter regions) were mostly phylogenetic variations, relating to the isolates' sequence types, whereas nonsynonymous SNPs inampDwere associated with derepression of AmpC and cephalosporin resistance. The additional loss of porins resulted in high-level carbapenem resistance, underlining the clinical importance of chromosomal mutations among carbapenem-resistantE. cloacae.
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