Evidence map›Paper›PMID 26856414›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2016

Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2.

Ayse Yildirim, Gulay Gulbol Duran, Nizami Duran, Kemal Jenedi, Behiye Sezgin Bolgul, Meral Miraloglu, Mustafa Muz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
11.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 126 citations in OpenAlex.

  1. Article
  2. Bee products as alternatives in the treatment of viral infections.Journal of the science of food and agriculture · 2026
    Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Natural antibiotics against antimicrobial resistance: sources and bioinspired delivery systems.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ayse YildirimDepartment of Histology and Embryology, Mustafa Kemal University, Medical Faculty, Hatay, Turkey.
Gulay Gulbol DuranDepartment of Medical Biology, Mustafa Kemal University, Medical Faculty, Hatay, Turkey.
Nizami DuranDepartment of Medical Microbiology, Mustafa Kemal University, Medical Faculty, Hatay, Turkey.
Kemal JenediDepartment of Medical Microbiology, Mustafa Kemal University, Medical Faculty, Hatay, Turkey.
Behiye Sezgin BolgulDepartment of Pedodontics, Mustafa Kemal University, Faculty of Dentistry, Hatay, Turkey.
Meral MiralogluDepartment of Medical Microbiology, Cukurova University, Medical Faculty, Adana, Turkey.
Mustafa MuzDepartment of Parasitology, Namık Kemal University, Veterinary Faculty, Tekirdag, Turkey.
Mustafa Kemal University · TRCukurova University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. MATERIAL AND METHODS All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1x105 cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 μg/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by real-time PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM). RESULTS The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 μg/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies. CONCLUSIONS We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propolis and acyclovir combined had a stronger effect against HSV-1 and HSV-2 than acyclovir alone.

Indexed as

AcyclovirAntiviral AgentsCell LineDrug SynergismHerpesvirus 1, HumanHerpesvirus 2, HumanHumansMedicine, TraditionalPropolisVirus ReplicationAcyclovirAntiviral AgentsPropolis

Identifiers

PMID26856414
PMCPMC4750782
OpenAlexW2259608716

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.