Evidence map›Paper›PMID 26855944›Full record

ArticleAnnals of translational medicine2016

Effects of methylene blue in acute lung injury induced by oleic acid in rats.

Ana Paula Cassiano Silveira, Daniella Alves Vento, Agnes Afrodite Sumarelli Albuquerque, Andrea Carla Celotto, Cristiane Tefé-Silva, Simone Gusmão Ramos, Tales Rubens de Nadai, Alfredo José Rodrigues, Omero Benedicto Poli-Neto, Paulo Roberto Barbosa Evora

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Article in Annals of translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ana Paula Cassiano Silveira1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Daniella Alves Vento1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Agnes Afrodite Sumarelli Albuquerque1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Andrea Carla Celotto1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Cristiane Tefé-Silva1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Simone Gusmão Ramos1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Tales Rubens de Nadai1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Alfredo José Rodrigues1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Omero Benedicto Poli-Neto1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Paulo Roberto Barbosa Evora1 Department of Surgery and Anatomy, 2 Department of Pathology, 3 Department of Gynecology and Obstetrics, Ribeirão Preto Faculty of Medicine, University of São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn acute lung injury (ALI), rupture of the alveolar-capillary barrier determines the protein-rich fluid influx into alveolar spaces. Previous studies have reported that methylene blue (MB) attenuates such injuries. This investigation was carried out to study the MB effects in pulmonary capillary permeability.

methodsWistar rats were divided into five groups: (I) Sham: saline bolus; (II) MB, MB infusion for 2 h; (III) oleic acid (OA), OA bolus; (IV) MB/OA, MB infusion for 2 h, and at 5 min after from the beginning, concurrently with an OA bolus; and (V) OA/MB, OA bolus, and after 2 h, MB infusion for 2 h. After 4 h, blood, bronchoalveolar lavage (BAL), and lung tissue were collected from all groups for analysis of plasma and tissue nitric oxide, calculation of the wet weight to dry weight ratio (WW/DW), and histological examination of lung tissue. Statistical analysis was performed using nonparametric test.

resultsAlthough favourable trends have been observed for permeability improvement parameters (WW/WD and protein), the results were not statistically significant. However, histological analysis of lung tissue showed reduced lesion areas in both pre- and post-treatment groups.

conclusionsThe data collected using this experimental model was favourable only through macroscopic and histological analysis. These observations are valid for both MB infusions before or after induction of ALI.

Indexed as

Acute lung injury (ALI)methylene blue (MB)oleic acid (OA)soluble guanylate cyclase (sGC)

Identifiers

PMID26855944
PMCPMC4716953

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.