Evidence map›Paper›PMID 26855741›Full record

ArticleJundishapur journal of microbiology2015

Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis.

Roohollah Fateh, Farideh Zaini, Parivash Kordbacheh, Mehraban Falahati, Sasan Rezaie, Roshanak Daie Ghazvini, Nahid Borhani, Mahin Safara, Azam Fattahi, Ali Kanani and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Jundishapur journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Structural and Functional Analysis of a Bidirectional Promoter fromInternational journal of molecular sciences · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Roohollah FatehDepartment of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, IR Iran; Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Farideh ZainiDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Parivash KordbachehDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Mehraban FalahatiDepartment of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
Sasan RezaieDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Roshanak Daie GhazviniDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Nahid BorhaniDepartment of Parasitology, Pasteur Institute of Iran, Tehran, IR Iran.
Mahin SafaraDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Azam FattahiDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Ali KananiDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
Shirin FarahyarDepartment of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
Manzar BolhassaniDepartment of Medical Genetics, Tehran University of Medical Sciences, Tehran, IR Iran.
Mansour HeidariDepartment of Medical Genetics, Tehran University of Medical Sciences, Tehran, IR Iran; Experimental Research Center, Tehran University of Medical Sciences, Tehran, IR Iran.
Tehran University of Medical Sciences · IRIran University of Medical Sciences · IRPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe production and development of an effective fungicidal drug requires the identification of an essential fungal protein as a drug target. Aconitase (ACO) is a mitochondrial protein that plays a vital role in tricarboxylic acid (TCA) cycle and thus production of energy within the cell.

objectivesThe current study aimed to sequence Candida krusei ACO gene and determine any amino acid residue differences between human and fungal aconitases to obtain selective inhibition. MATERIALS AND

methodsCandida krusei (ATCC: 6258) aconitase gene was determined by 5'Rapid Amplification of cDNA Ends (RACE) method and degenerate Polymerase Chain Reaction (PCR) and analyzed using bioinformatics softwares.

resultsOne thousand-four hundred-nineteen nucleotide of C. krusei aconitase gene were clarified and submitted in Genbank as a partial sequence and then taxonomic location of C. krusei was determined by nucleotide and amino acid sequences of this gene. The comparison of nucleotide and amino acid sequences of Candida species ACO genes showed that C. krusei possessed characteristic sequences. No significant differences were observed between C. krusei and human aconitases within the active site amino acid residues.

conclusionsResults of the current study indicated that aconitase was not a suitable target to design new anti-fungal drugs that selectively block this enzyme.

Indexed as

5’ RACE MethodAconitate HydrataseCandida kruseiDegenerate PCRPhylogenetic Analysis

Identifiers

PMID26855741
PMCPMC4736022
OpenAlexW2180552373

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.