ArticleAvicenna journal of medical biotechnology
The Effect of Angiotensin on the Quality of In Vitro Produced (IVP) Sheep Embryos and Expression of Na(+)/K(+)/ATPase.
Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Reactive Oxygen Species in Follicular Fluid as a Potential Biomarker of Oocyte Developmental Competence.Journal of reproduction & infertility · 2025Article
- Stimulation of NaOxidative medicine and cellular longevity · 2019Article
- The Effect of Media Supplementation with Angiotensin on Developmental Competence of Ovine Embryos Derived from Vitrified-warmed Oocytes.Avicenna journal of medical biotechnologyArticle
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe presence of rennin-angiotensin components in mammalian ovaries and their involvement in ovarian physiology have been established. In the present study, effects of angiotensin II (Ang II) on sodium-potassium adenosine triphosphatase (Na(+)/K(+)/ATPase) expression and development of sheep embryos was evaluated.
methodsThe abattoir-derived Cumulus Oocyte Complexes (COC) were randomly allocated into three experimental groups; group I) in vitro Maturation (IVM) of oocytes in the presence of Ang II followed by in vitro fertilization (IVF)/in vitro Culture (IVC) (IVM group), group II) IVM/IVF of oocytes followed by IVC wherein the embryos were exposed to Ang II on day 4 of IVC (D4 group), and group III) IVM/IVF and IVC of oocytes without any angiotensin (Control). The blastocyst and hatching rates were recorded on days 6 to 8. Day 8 embryos were immunostained with primary and secondary antibodies against Na(+)/K(+)/ATPase α1 and β1 subunits.
resultsAddition of Ang II during IVM and IVC significantly increased the hatching rate of blastocysts on day 8 compared to the control. The trophectoderm and total blastocyst cells' numbers were significantly increased by addition of Ang II to the IVM and IVC media, though the expression of Na(+)/K(+)/ATPase α1 and β1 subunits were positively influenced by the addition of Ang II on day 4 (D4 group).
conclusionIn conclusion, it seems Ang II through positive effects on embryos, expressed as the greater hatching rate and blastocyst cell number, could increase the sheep embryo developmental rate. These improvements might be partly related to the greater expression of Na(+)/K(+)/ATPase α1 and β1 subunits when Ang II was added during IVC.
Indexed as
Identifiers
26855730PMC4717467W2267841323What OpenQuestion holds
Registered trials
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