ArticleMolecular and cellular biology1989
A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- cis Elements that Mediate RNA Polymerase II Pausing Regulate Human Gene Expression.American journal of human genetics · 2019Article
- Role of CXCL1 in tumorigenesis of melanoma.Journal of leukocyte biology · 2002Review
- The role of CDP in the negative regulation of CXCL1 gene expression.The Journal of biological chemistry · 2001Article
- Nucleosomal structures of c-myc promoters with transcriptionally engaged RNA polymerase II.Molecular and cellular biology · 1997Article
- Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts.Molecular and cellular biology · 1997Article
- TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.Molecular and cellular biology · 1996Article
- Dietary marine lipids suppress continuous expression of interleukin-1 beta gene transcription.Lipids · 1996Article
- A Myc-associated zinc finger protein binding site is one of four important functional regions in the CD4 promoter.Molecular and cellular biology · 1995Article
- MAZ-dependent termination between closely spaced human complement genes.The EMBO journal · 1994Article
- Down-regulation of Rous sarcoma virus long terminal repeat promoter activity by a HeLa cell basic protein.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- A transcriptional silencer controls the developmental expression of the CD4 gene.The EMBO journal · 1994Article
- Dual modes of control of c-fos mRNA induction by intracellular calcium in T cells.Molecular and cellular biology · 1994Article
- The human cut homeodomain protein represses transcription from the c-myc promoter.Molecular and cellular biology · 1994Article
- Premature termination of tubulin gene transcription in Xenopus oocytes is due to promoter-dependent disruption of elongation.Molecular and cellular biology · 1993Article
- Distinct properties of c-myc transcriptional elongation are revealed in Xenopus oocytes and mammalian cells and by template titration, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), and promoter mutagenesis.Molecular and cellular biology · 1993Article
- Repeated CT elements bound by zinc finger proteins control the absolute and relative activities of the two principal human c-myc promoters.Molecular and cellular biology · 1993Article
- Regulation of eukaryotic gene expression by transcriptional attenuation.Molecular biology of the cell · 1993Review
- Expression of c-fos and c-myc protooncogenes in an immortalized hepatocyte line harbouring SV40 T antigen and hGH as transgenes.Transgenic research · 1993Article
- Transcription elongation in the human c-myc gene is governed by overall transcription initiation levels in Xenopus oocytes.Molecular and cellular biology · 1993Article
- Sequences in the human c-myc P2 promoter affect the elongation and premature termination of transcripts initiated from the upstream P1 promoter.Molecular and cellular biology · 1992Article
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Authors and funding
7 authors.
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Abstract
A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The transcription start sites were determined by S1 nuclease mapping analysis, and the extent of transcriptional block was measured by nuclear run-on transcription assays. Our results demonstrate that at least two cis-acting elements are necessary for the transcriptional block. A 3' element was found to be located in the region where transcription stopped and showed features reminiscent of some termination sites found in procaryotes. A 5' element was positioned between the P1 and P2 (C. Asselin, A. Nepveu, and K. B. Marcu, Oncogene 4:549-558, 1989). Removal of the more 3' binding site abolished the transcriptional block.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.