ArticleLaboratory investigation; a journal of technical methods and pathology2016
Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair.
Article in Laboratory investigation; a journal of technical methods and pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Guideline
- Yoda1-activated Piezo1 enhances mitophagy in human gingival fibroblasts to promote maxillofacial wound repair.Frontiers in pharmacology · 2026Article
- Activated carbon-chitosan hydrogel dressing loaded with LL37 microspheres for the treatment of infected wounds: In vivo antimicrobial and antitoxin assessment.Drug delivery and translational research · 2025Article
- Pinitol Improves Lipopolysaccharide-Induced Cellular Damage in Human Dermal Microvascular Endothelial Cells.Molecules (Basel, Switzerland) · 2025Article
- Article
- Estrogen and bacterial infection.Frontiers in immunology · 2025Review
- Article
- Candidiasis in Pregnancy: Relevant Aspects of the Pathology for the Mother and the Fetus and Therapeutic Strategies.Tropical medicine and infectious disease · 2024Review
- Development of phage-containing hydrogel for treating Enterococcus faecalis-infected wounds.PloS one · 2024Article
- DNA methyltransferase 1 deficiency improves macrophage motility and wound healing by ameliorating cholesterol accumulation.NPJ Regenerative medicine · 2023Article
- Estrogen-sensitive activation of SGK1 induces M2 macrophages with anti-inflammatory properties and a Th2 response at the maternal-fetal interface.Reproductive biology and endocrinology : RB&E · 2023Article
- Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis.The Kaohsiung journal of medical sciences · 2023Article
- An in vivo critically colonised wound model with dysbiotic wound microbiota.International wound journal · 2023Article
- Point-of-care fluorescence imaging reveals extent of bacterial load in diabetic foot ulcers.International wound journal · 2023Article
- Functional responses of dermal fibroblasts to low nutrition and pro-inflammatory stimuli mimicking a wound environment in vitro.In vitro cellular & developmental biology. Animal · 2022Article
- Effect of samarium oxide nanoparticles on virulence factors and motility of multi-drug resistant Pseudomonas aeruginosa.World journal of microbiology & biotechnology · 2022Article
- Proteomic Profiling of Outer Membrane Vesicles Released byJournal of personalized medicine · 2022Article
- SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy.Journal of nanobiotechnology · 2022Article
- Genital Wound Repair and Scarring.Medical sciences (Basel, Switzerland) · 2022Review
- The Impact of Prolonged Inflammation on Wound Healing.Biomedicines · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Wound infection is a major clinical problem, yet understanding of bacterial host interactions in the skin remains limited. Microbe-derived molecules, known as pathogen-associated molecular patterns, are recognised in barrier tissues by pattern-recognition receptors. In particular, the pathogen-associated molecular pattern, lipopolysaccharide (LPS), a component of microbial cell walls and a specific ligand for Toll-like receptor 4, has been widely used to mimic systemic and local infection across a range of tissues. Here we administered LPS derived from Klebsiella pneumoniae, a species of bacteria that is emerging as a wound-associated pathogen, to full-thickness cutaneous wounds in C57/BL6 mice. Early in healing, LPS-treated wounds displayed increased local apoptosis and reduced proliferation. Subsequent healing progression was delayed with reduced re-epithelialisation, increased proliferation, a heightened inflammatory response and perturbed wound matrix deposition. Our group and others have previously demonstrated the beneficial effects of 17β-estradiol treatment across a range of preclinical wound models. Here we asked whether oestrogen would effectively promote healing in our LPS bacterial infection model. Intriguingly, co-treatment with 17β-estradiol was able to promote re-epithelialisation, dampen inflammation and induce collagen deposition in our LPS-delayed healing model. Collectively, these studies validate K. pneumoniae-derived LPS treatment as a simple yet effective model of bacterial wound infection, while providing the first indication that oestrogen could promote cutaneous healing in the presence of infection, further strengthening the case for its therapeutic use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.