ArticleAnalytical chemistry2016
Metabolite Profiling and Stable Isotope Tracing in Sorted Subpopulations of Mammalian Cells.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Metabolomics of in vivo inflammation identifies soluble sialic acid as a conserved myeloid-cell metabolite.The Journal of biological chemistry · 2026Article
- Metabolomics ofbioRxiv : the preprint server for biology · 2026Article
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- Acidity suppresses CD8 + T-cell function by perturbing IL-2, mTORC1, and c-Myc signaling.The EMBO journal · 2024Article
- Multiomics Characterization of a Less Invasive Microfluidic-Based Cell Sorting Technique.Journal of proteome research · 2024Article
- A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella.PLoS biology · 2023Article
- PERK is a critical metabolic hub for immunosuppressive function in macrophages.Nature immunology · 2022Article
- Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors.Science advances · 2022Article
- Untargeted stable isotope-resolved metabolomics to assess the effect of PI3Kβ inhibition on metabolic pathway activities in a PTEN null breast cancer cell line.Frontiers in molecular biosciences · 2022Article
- Metabolome Shift in Both Metastatic Breast Cancer Cells and Astrocytes Which May Contribute to the Tumor Microenvironment.International journal of molecular sciences · 2021Article
- Comparing Stable Isotope Enrichment by Gas Chromatography with Time-of-Flight, Quadrupole Time-of-Flight, and Quadrupole Mass Spectrometry.Analytical chemistry · 2021Article
- Adenylosuccinate lyase is oncogenic in colorectal cancer by causing mitochondrial dysfunction and independent activation of NRF2 and mTOR-MYC-axis.Theranostics · 2021Article
- Mapping metabolic oscillations during cell cycle progression.Cell cycle (Georgetown, Tex.) · 2020Article
- Article
- Microfluidic single-cell analysis-Toward integration and total on-chip analysis.Biomicrofluidics · 2020Review
- Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarizedScientific reports · 2020Article
- Mapping Metabolic Events in the Cancer Cell Cycle Reveals Arginine Catabolism in the Committed SGCell reports · 2019Article
- Metabolic reprogramming of acute lymphoblastic leukemia cells in response to glucocorticoid treatment.Cell death & disease · 2018Article
- Sorting cells alters their redox state and cellular metabolome.Redox biology · 2018Article
- A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Biological samples such as tissues, blood, or tumors are often complex and harbor heterogeneous populations of cells. Separating out specific cell types or subpopulations from such complex mixtures to study their metabolic phenotypes is challenging because experimental procedures for separation may disturb the metabolic state of cells. To address this issue, we developed a method for analysis of cell subpopulations using stable isotope tracing and fluorescence-activated cell sorting followed by liquid chromatography-high-resolution mass spectrometry. To ensure a faithful representation of cellular metabolism after cell sorting, we benchmarked sorted extraction against direct extraction. While peak areas differed markedly with lower signal for amino acids but higher signal for nucleotides, mass isotopomer distributions from sorted cells were generally in good agreement with those obtained from direct extractions, indicating that they reflect the true metabolic state of cells prior to sorting. In proof-of-principle studies, our method revealed metabolic phenotypes specific to T cell subtypes, and also metabolic features of cells in the committed phase of the cell division cycle. Our approach enables studies of a wide range of adherent and suspension cell subpopulations, which we anticipate will be of broad importance in cell biology and biomedicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.