ArticleLife sciences2016
Constitutive expression Of NGF And P75(NTR) affected by bladder distension and NGF antisense treatment.
Article in Life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Caffeine improves bladder function in diabetic rats via a neuroprotective effect.Experimental and therapeutic medicine · 2021Article
- Expression of Low Affinity Nerve Growth Factor Receptor p75 in Classic Bladder Exstrophy.Frontiers in pediatrics · 2021Article
- Partners in Crime: NGF and BDNF in Visceral Dysfunction.Current neuropharmacology · 2019Review
- Drug Targets in Neurotrophin Signaling in the Central and Peripheral Nervous System.Molecular neurobiology · 2018Review
- BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity.American journal of physiology. Renal physiology · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
aimsIt is known that bladder exposure to noxious stimuli elicits nerve growth factor (NGF) expression with region wise differences. Here, we investigated the effect of bladder distension (cystometry) and bladder wall injection of NGF antisense oligonucleotide (ODN) together as well as separately on spontaneous (constitutive) expression of NGF and its cognate p75 neurotrophin receptor (p75(NTR)).
methodUnder isoflurane anesthesia, either 15μg of protamine sulfate (vehicle) alone or complexed with 1.5μg of NGF antisense or scrambled ODN was injected (10μL) at 4 sites in bladder wall of 24 adult female Sprague-Dawley rats and 6 rats were left untreated (n=30). Under urethane anesthesia, cystometry (CMG) was performed in treated and control rats. Fluorescent ODN and NGF/p75(NTR) expression was localized in harvested tissue. KEY
findingsComplexation of ODN with protamine was essential for the retention of ODN in bladder tissue as the uncomplexed ODN was untraceable after injection. Bladder distension from CMG raised the expression of NGF and p75(NTR) relative to CMG naïve rats. The groups treated with vehicle, scrambled and antisense ODN were indistinct with regard to CMG parameters, but the intense immunoreactivity of NGF and p75(NTR) seen in the vehicle and scrambled ODN groups was reduced following treatment with NGF antisense. SIGNIFICANCE: The constitutive expression of NGF and p75(NTR) is responsive to bladder distension and administration of NGF antisense. Complexation with protamine reduces the clearance of ODN and demonstrates the potential of ODN nanoparticles as an option for reducing the inducible NGF expression in OAB patients following intradetrusor injection.
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