ArticleNature chemical biology2016
A transcription activator-like effector (TALE) induction system mediated by proteolysis.
Article in Nature chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- LOTUS: A low-cost time-lapse automated imaging system for spatio-temporal analysis of microbial colony or biofilm development.PloS one · 2026Article
- Subunits of an E3 Ligase Complex as Degrons for Efficient Degradation of Cytosolic, Nuclear, and Membrane Proteins.ACS synthetic biology · 2024Article
- Reprogramming the Cleavage Specificity of Botulinum Neurotoxin Serotype B1.ACS synthetic biology · 2022Article
- A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.Nucleic acids research · 2019Article
- Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta.Plant physiology · 2019Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway.Metabolic engineering · 2018Article
- Selective recognition ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Engineered promoters enable constant gene expression at any copy number in bacteria.Nature biotechnology · 2018Article
- Sequence-based prediction of permissive stretches for internal protein tagging and knockdown.BMC biology · 2017Article
- Transcription control engineering and applications in synthetic biology.Synthetic and systems biotechnology · 2017Review
- Evolution of a split RNA polymerase as a versatile biosensor platform.Nature chemical biology · 2017Article
- Genome-Editing Technologies: Principles and Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Optimization of the Production of Soluble Recombinant TEV Protease in TwoAvicenna journal of medical biotechnologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Simple and predictable trans-acting regulatory tools are needed in the fields of synthetic biology and metabolic engineering to build complex genetic circuits and optimize the levels of native and heterologous gene products. Transcription activator-like effectors (TALEs) are bacterial virulence factors that have recently gained traction in biotechnology applications owing to their customizable DNA-binding specificity. In this work we expanded the versatility of these transcription factors to create an inducible TALE system by inserting tobacco-etch virus (TEV) protease recognition sites into the TALE backbone. The resulting engineered TALEs maintain transcriptional repression of their target genes in Escherichia coli, but are degraded after induction of the TEV protease, thereby promoting expression of the previously repressed target gene of interest. This TALE-TEV technology enables both repression and induction of plasmid or chromosomal target genes in a manner analogous to traditional repressor proteins but with the added flexibility of being operator-agnostic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.