ArticleThe Journal of veterinary medical science2016
Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses.
Article in The Journal of veterinary medical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Isolation and genetic characterization of waterfowl parvovirus in ducks in Northern Vietnam.Veterinary world · 2024Article
- Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3.Frontiers in cellular and infection microbiology · 2024Article
- Specific detection and differentiation of classic goose parvovirus and novel goose parvovirus by TaqMan real-time PCR assay, coupled with host specificity.BMC veterinary research · 2019Article
- Development of a PCR assay for detection and differentiation of Muscovy duck and goose parvoviruses based on NS gene characterization.The Journal of veterinary medical science · 2018Article
- Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay.BMC veterinary research · 2018Article
- A novel group of avian Avastrovirus in domestic geese, China.The Journal of veterinary medical science · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR.
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