ArticleThe Journal of reproduction and development2016
Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone β-subunit gene.
Article in The Journal of reproduction and development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Effects of aqueous extract from Cyathula prostrata (Linn.) Blume (Amaranthaceae) on puberty onset and some reproductive parameters in immature female Wistar rats.Hormones (Athens, Greece) · 2025Article
- Enhancement of Female Rat Fertility via Ethanolic Extract fromMolecules (Basel, Switzerland) · 2024Article
- Effects of dietary Enteromorpha powder on reproduction-related hormones and genes during the late laying period of Zi geese.Animal bioscience · 2021Article
- Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids.The Journal of reproduction and development · 2020Article
- Short-term but not long-term high-fat diet induces an increase in gene expression of gonadotropic hormones and GPR120 in the male mouse pituitary gland.The Journal of reproduction and development · 2020Article
- AMP-activated protein kinase activation reduces the transcriptional activity of the murine luteinizing hormone β-subunit gene.The Journal of reproduction and development · 2020Article
- Colocalization of GPR120 and anterior pituitary hormone-producing cells in female Japanese Black cattle.The Journal of reproduction and development · 2020Article
- Activation of GPR40 Suppresses AGE-Induced Reduction of Type II Collagen and Aggrecan in Human SW1353 Chondrocytes.Drug design, development and therapy · 2020Article
- Reduction in FSH Throughout the Menstrual Cycle After Omega-3 Fatty Acid Supplementation in Young Normal Weight but not Obese Women.Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Molecular regulation of follicle-stimulating hormone synthesis, secretion and action.Journal of molecular endocrinology · 2018Review
- Transcription of follicle-stimulating hormone subunit genes is modulated by porcine LIM homeobox transcription factors, LHX2 and LHX3.The Journal of reproduction and development · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we assessed the effects of long-chain fatty acids (LCFAs) and the LCFA receptor agonist GW9508 on the transcription of the gonadotropin subunit genes Cga, Lhb and Fshb because LCFA receptor GPR120 was observed in mouse gonadotropes in our recent study. A transcription assay using LβT2 cells demonstrated that LCFAs, oleic acid, α-linolenic acid, docosahexaenoic acid and palmitate, repressed the expression of Cga, Lhb, and Fshb at concentrations between 50 and 100 µM. On the other hand, treatment with 10 µM unsaturated LCFAs, oleic acid, α-linolenic acid and docosahexaenoic acid, repressed only Fshb expression, while the same dose of a saturated LCFA, palmitate, had no effect on the expression of gonadotropin subunit genes. Furthermore, GW9508 did not affect promoter activity. Next, we examined deletion mutants of the upstream region of Fshb and found that the upstream regulatory region (-2824 to -2343 bp) of Fshb was responsible for the notable repression by 10 µM unsaturated LCFAs. Our results suggest that the upstream region of Fshb is susceptible to unsaturated LCFAs. In addition, unsaturated LCFAs play a role in repressing Fshb expression through the distal -2824 to -2343 bp region, which might be independent of the LCFA receptor GPR120 pathway.
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