ArticleScientific reports2016
Development and cross-species transferability of EST-SSR markers in Siberian wildrye (Elymus sibiricus L.) using Illumina sequencing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed.
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- Screening and identification of multiple abiotic stress responsive candidate genes based on hybrid-sequencing inHeliyon · 2023Article
- Characterization and Application of EST-SSR Markers Developed from Transcriptome Sequences inGenes · 2023Article
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- Analysis of Genetic Diversity of Fescue Populations from the Highlands of Bolivia Using EST-SSR Markers.Genes · 2022Article
- A pipeline for effectively developing highly polymorphic simple sequence repeats markers based on multi-sample genomic data.Ecology and evolution · 2022Article
- Phylogeographical Pattern and Population Evolution History of IndigenousFrontiers in plant science · 2022Article
- Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers inFrontiers in plant science · 2022Article
- Full-Length Transcriptomics Reveals Complex Molecular Mechanism of Salt Tolerance inFrontiers in plant science · 2022Article
- RNA-Seq Provides Novel Genomic Resources for Noug (Frontiers in plant science · 2022Article
- Transcriptome Analysis of Salt Stress inInternational journal of molecular sciences · 2021Article
- Genetic diversity and population structure analysis in a large collection of Vicia amoena in China with newly developed SSR markers.BMC plant biology · 2021Article
- Transcriptomic resources for prairie grass (Bromus catharticus): expressed transcripts, tissue-specific genes, and identification and validation of EST-SSR markers.BMC plant biology · 2021Article
- De novo transcriptomic analysis and identification of EST-SSR markers in Stephanandra incisa.Scientific reports · 2021Article
- Genomic survey sequencing, development and characterization of single- and multi-locus genomic SSR markers of Elymus sibiricus L.BMC plant biology · 2021Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Siberian wildrye (Elymus sibiricus L.) is a perennial, self-fertilizing grass that plays an important role in animal husbandry and environmental sustenance. However, the transcriptomic and genomic information on this species is very limited, which hinders genetic and breeding studies. In the present study, 76,686,804 clean reads were generated from 11 different tissue samples of E. sibiricus by Illumina paired-end sequencing, and the reads were deposited into the NCBI SRA database (SRX574376). A total of 8,769 EST-SSRs were identified from 94,458 unigene sequences, which were obtained by de novo assembly. Moreover, 1,078 primer pairs were successfully designed, and 500 pairs were randomly selected to assess polymorphisms in 15 E. sibiricus accessions. A total of 112 primer pairs were polymorphic, and the polymorphism information content (PIC) values ranged from 0.39 to 0.81, indicating a high level of informativeness. Furthermore, these 112 polymorphic primer pairs were used to evaluate the transferability to 13 other related species, and 55 EST-SSR markers were found to be polymorphic among these 13 Elymus species. This study collected the global sequence data for E. sibiricus, and the newly developed markers will prove valuable in facilitating genetic diversity in E. sibiricus and related Elymus species.
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