ArticleStem cell reports2016
Dnd Is a Critical Specifier of Primordial Germ Cells in the Medaka Fish.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.Stem cell reviews and reports · 2026Review
- Article
- Direct male development in chromosomally ZZ zebrafish.Frontiers in cell and developmental biology · 2024Article
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- Spinyhead Croaker Germ Cells GeneLife (Basel, Switzerland) · 2022Article
- Article
- Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (Life (Basel, Switzerland) · 2022Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
- Dead-end (dnd) protein in fish-a review.Fish physiology and biochemistry · 2021Review
- Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.Scientific reports · 2020Article
- Germline Specific Expression of aFrontiers in cell and developmental biology · 2020Article
- Non-coding RNA regulation in reproduction: Their potential use as biomarkers.Non-coding RNA research · 2019Review
- Heat shock factor 1 protects germ cell proliferation during early ovarian differentiation in medaka.Scientific reports · 2019Article
- A High-Density Genetic Linkage Map and QTL Mapping for Sex and Growth-Related Traits of Large-Scale Loach (Frontiers in genetics · 2019Article
- Germline replacement by blastula cell transplantation in the fish medaka.Scientific reports · 2016Article
- Dazl is a critical player for primordial germ cell formation in medaka.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primordial germ cell (PGC) specification occurs early in development. PGC specifiers have been identified in Drosophila, mouse, and human but remained elusive in most animals. Here we identify the RNA-binding protein Dnd as a critical PGC specifier in the medaka fish (Oryzias latipes). Dnd depletion specifically abolished PGCs, and its overexpression boosted PGCs. We established a single-cell culture procedure enabling lineage tracing in vitro. We show that individual blastomeres from cleavage embryos at the 32- and 64-cell stages are capable of PGC production in culture. Importantly, Dnd overexpression increases PGCs via increasing PGC precursors. Strikingly, dnd RNA forms prominent particles that segregate asymmetrically. Dnd concentrates in germ plasm and stabilizes germ plasm RNA. Therefore, Dnd is a critical specifier of fish PGCs and utilizes particle partition as a previously unidentified mechanism for asymmetric segregation. These findings offer insights into PGC specification and manipulation in medaka as a lower vertebrate model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.