ArticleScientific reports2016
Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 77 citations in OpenAlex.
- Beyond conventional imaging: curvelet-AI convergence for non-invasive microalgal lipid quantification and biofuel optimization.Bioprocess and biosystems engineering · 2026Review
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- Phototactic microswimmers in pulsatile flow: Toward a novel harvesting method.Biomicrofluidics · 2022Article
- Improved Productivity of Astaxanthin from PhotosensitiveMarine drugs · 2022Article
- Article
- The Fusion of Microfluidics and Optics for On-Chip Detection and Characterization of Microalgae.Micromachines · 2021Review
- Comparative analysis of freshwater phytoplankton communities in two lakes of Burabay National Park using morphological and molecular approaches.Scientific reports · 2021Article
- Microfluidic techniques for separation of bacterial cells via taxis.Microbial cell (Graz, Austria) · 2020Review
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Development and characterization of aBiotechnology for biofuels · 2020Article
- Microfluidic tools for lipid production and modification: a review.Environmental science and pollution research international · 2019Review
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
- Microfluidic technology for plankton research.Current opinion in biotechnology · 2019Review
- Biomass from microalgae: the potential of domestication towards sustainable biofactories.Microbial cell factories · 2018Review
- Phototaxis Assay forBio-protocol · 2017Article
- Phototaxis beyond turning: persistent accumulation and response acclimation of the microalga Chlamydomonas reinhardtii.Scientific reports · 2017Article
- Quantitative analysis of the chemotaxis of a green alga, Chlamydomonas reinhardtii, to bicarbonate using diffusion-based microfluidic device.Biomicrofluidics · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae possess great potential as a source of sustainable energy, but the intrinsic inefficiency of photosynthesis is a major challenge to realize this potential. Photosynthetic organisms evolved phototaxis to find optimal light condition for photosynthesis. Here we report a microfluidic screening using competitive phototaxis of the model alga, Chlamydomonas reinhardtii, for rapid isolation of strains with improved photosynthetic efficiencies. We demonstrated strong relationship between phototaxis and photosynthetic efficiency by quantitative analysis of phototactic response at the single-cell level using a microfluidic system. Based on this positive relationship, we enriched the strains with improved photosynthetic efficiency by isolating cells showing fast phototactic responses from a mixture of 10,000 mutants, thereby greatly improving selection efficiency over 8 fold. Among 147 strains isolated after screening, 94.6% showed improved photoautotrophic growth over the parental strain. Two mutants showed much improved performances with up to 1.9- and 8.1-fold increases in photoautotrophic cell growth and lipid production, respectively, a substantial improvement over previous approaches. We identified candidate genes that might be responsible for fast phototactic response and improved photosynthesis, which can be useful target for further strain engineering. Our approach provides a powerful screening tool for rapid improvement of microalgal strains to enhance photosynthetic productivity.
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