ArticleThe Journal of veterinary medical science2016
Evaluation of response to restraint stress by salivary corticosterone levels in adult male mice.
Article in The Journal of veterinary medical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Stress estimation by serum cortisol release during rapid maxillary expansion in mice: validation of an experimental model.Dental press journal of orthodontics · 2026Article
- Comparison of hair steroid levels among Wistar rats exposed to different environmental enrichment settings.Veterinary world · 2024Article
- Restraint Stress-Induced Neutrophil Inflammation Contributes to Concurrent Gastrointestinal Injury in Mice.International journal of molecular sciences · 2024Article
- Neurotoxic stimulation alters prosaposin levels in the salivary systems of rats.Cell and tissue research · 2024Article
- Review of Rat (Animals : an open access journal from MDPI · 2023Review
- Determination of diurnal rhythm of salivary corticosterone concentration in the domestic rabbit (Oryctolagus cuniculus f. domestica) using a non-invasive method.Scientific reports · 2023Article
- Selection of appropriate biomatrices for studies of chronic stress in animals: a review.Journal of animal science and technology · 2022Review
- Anxiolytic Effect of Two Tobacco Essential Oils (Molecules (Basel, Switzerland) · 2021Article
- Sleep loss mediates the effect of stress on nitrergic signaling in female mice.Neuroscience letters · 2021Article
- Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice.Animals : an open access journal from MDPI · 2020Article
- Chronic unpredictable stress-induced inflammation and quantitative analysis of neurons of distinct brain regions in Wistar rat model of comorbid depression.Veterinary world · 2020Article
- Does environmental stress affect cortisol biodistribution in freshwater mussels?Conservation physiology · 2019Article
- A refinement to the formalin test in mice.F1000Research · 2019Article
- Restraint stress in lactating mice alters the levels of sulfur-containing amino acids in milk.The Journal of veterinary medical science · 2018Article
- Premedication with fentanyl-midazolam improves sevoflurane anesthesia for surgical intervention in laboratory mice.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Evidence for deficits in behavioural and physiological responses in aged mice relevant to the psychiatric symptom of apathy.Brain and neuroscience advancesArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saliva as a sampling method is a low invasive technique for the detection of physiologically active substances, as opposed to sampling the plasma or serum. In this study, we obtained glucocorticoids transferred from the blood to the saliva from mice treated with 2.0 mg/kg via an intraperitoneal injection of cortisol. Next, to evaluate the effect of restraint stress using mouse saliva-collected under anesthesia by mixed anesthetic agents-we measured plasma and salivary corticosterone levels at 60 min after restraint stress. Moreover, to evaluate salivary corticosterone response to stress in the same individual mouse, an adequate recovery period (1, 3 and 7 days) after anesthesia was examined. The results demonstrate that exogenous cortisol was detected in the saliva and the plasma, in mice treated with cortisol. Restraint stress significantly increased corticosterone levels in both the plasma and saliva (P<0.001). Monitoring the results of individual mice showed that restraint stress significantly increased salivary corticosterone levels in all three groups (1-, 3- and 7-day recovery). However, the statistical evidence of corticosterone increase is stronger in the 7-day recovery group (P<0.001) than in the others (P<0.05). These results suggest that the corticosterone levels in saliva reflect its levels in the plasma, and salivary corticosterone is a useful, less-invasive biomarker of physical stress in mice. The present study may contribute to concepts of Reduction and Refinement of the three Rs in small animal experiments.
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