ArticleJournal of assisted reproduction and genetics2016
A novel technique for in vitro maturation of sheep oocytes in a liquid marble microbioreactor.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Advanced three-dimensional culture technologies in reproductive medicine: A narrative review.International journal of reproductive biomedicine · 2026Review
- Effect of Liquid Marble 3D Culture System on In Vitro Maturation and Embryo Development of Prepubertal Goat Oocytes.Animals : an open access journal from MDPI · 2025Article
- 3D culture applied to reproduction in females: possibilities and perspectives.Animal reproduction · 2024Review
- Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.Animals : an open access journal from MDPI · 2023Article
- IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture.International journal of molecular sciences · 2023Article
- New Technique for Human Sperm Cryopreservation Using Emptied Sheep's Ovarian Follicles.Archives of Razi Institute · 2023Article
- Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.PloS one · 2023Article
- Embryo biotechnologies in sheep: Achievements and new improvements.Reproduction in domestic animals = Zuchthygiene · 2022Review
- Liquid marbles, floating droplets: preparations, properties, operations and applications.RSC advances · 2022Review
- Microtubular Assessment of C6 Rat Glioma Cell Spheroids Developed in Transparent Liquid Marbles or Hanging Drops.Biology · 2022Article
- Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends.Biosensors · 2021Review
- 3D Liquid Marble Microbioreactors SupportBiology · 2021Article
- Fighting Like Cats and Dogs: Challenges in Domestic Carnivore Oocyte Development and Promises of Innovative Culture Systems.Animals : an open access journal from MDPI · 2021Review
- Article
- Current Advances in 3D Tissue and Organ Reconstruction.International journal of molecular sciences · 2021Review
- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Liquid Marbles: From Industrial to Medical Applications.Molecules (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this work was to develop a microbioreactor using liquid marble (LM) as a novel system for oocyte in vitro maturation (IVM) in small volumes.
methodsCumulus-oocyte complexes (COCs) obtained from slaughterhouse sheep ovaries were in vitro matured in a LM system prepared by placing a drop (30 μl containing 10 COCs) suspended in TCM 199 supplemented with 10 % (v/v) oestrus sheep serum (OSS) and 0.1 IU FSH and LH onto a polytetrafluoroethylene (PTFE) particle bed (LM group). As a control group (CTRL group), COCs were in vitro matured in standard volume and conditions (600 μl of IVM medium in a four-well dish). After 24-h culture at 38.5 °C in 5 % CO2 in air, COCs were released from LM and the following parameters were evaluated: (a) percentage of MII oocytes, (b) oocyte developmental competence following in vitro fertilization (IVF) or parthenogenetic activation (PA) and embryo culture for 8 days in synthetic oviductal fluid (SOF) medium at 38.5 °C in 5 % O2, 5 % CO2, and 90 % N2.
resultsThe results indicated similar percentage of MII oocytes in LM and CTRL groups (88.0 vs. 92.0 %). No differences were observed in blastocyst rate after IVF (LM 47.5 % vs. CTRL 50.2 %, P=0.637) or PA (LM 44.4 % vs. CTRL 48.3 %, P=0.426).
conclusionsThe results indicate that LM microbioreactor is a viable technique that provides a suitable microenvironment to induce oocyte in vitro maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.