ArticleCardiovascular diabetology2016
Type 2 diabetes enhances arterial uptake of choline in atherosclerotic mice: an imaging study with positron emission tomography tracer ¹⁸F-fluoromethylcholine.
Article in Cardiovascular diabetology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Radiopharmaceuticals for PET and SPECT Imaging: A Literature Review over the Last Decade.International journal of molecular sciences · 2022Pooled it
- From incidental positron emission uptake toFrontiers in cardiovascular medicine · 2026Review
- Choline in immunity: a key regulator of immune cell activation and function.Frontiers in immunology · 2025Review
- Integrated multi-omics analysis reveals that Gongying San ameliorates subclinical mastitis by modulating intestinal microbiota and metabolites in dairy cows.Frontiers in veterinary science · 2025Article
- The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation.Cell proliferation · 2023Article
- Dietary NWorld journal of diabetes · 2022Article
- Atherosclerosis Burdens in Diabetes Mellitus: Assessment by PET Imaging.International journal of molecular sciences · 2022Review
- Positron Emission Tomography in Atherosclerosis Research.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Scientific Advances in Diabetes: The Impact of the Innovative Medicines Initiative.Frontiers in medicine · 2021Review
- Novel Positron Emission Tomography Tracers for Imaging Vascular Inflammation.Current cardiology reports · 2020Review
- Current and novel radiopharmaceuticals for imaging cardiovascular inflammation.The quarterly journal of nuclear medicine and molecular imaging : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society of... · 2020Review
- Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.Cell metabolism · 2019Article
- Can Metabolic Pathways Be Therapeutic Targets in Rheumatoid Arthritis?Journal of clinical medicine · 2019Review
- Current and Emerging Preclinical Approaches for Imaging-Based Characterization of Atherosclerosis.Molecular imaging and biology · 2018Review
- Aluminum fluoride-18 labeled folate enables in vivo detection of atherosclerotic plaque inflammation by positron emission tomography.Scientific reports · 2018Article
- New methods to image unstable atherosclerotic plaques.Atherosclerosis · 2018Review
- Positron Emission Tomography Imaging of Macrophages in Atherosclerosis withContrast media & molecular imaging · 2018Article
- Altered glucose metabolism and hypoxic response in alloxan-induced diabetic atherosclerosis in rabbits.PloS one · 2017Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiabetes is a risk factor for atherosclerosis associated with oxidative stress, inflammation and cell proliferation. The purpose of this study was to evaluate arterial choline uptake and its relationship to atherosclerotic inflammation in diabetic and non-diabetic hypercholesterolemic mice.
methodsLow-density lipoprotein-receptor deficient mice expressing only apolipoprotein B100, with or without type 2 diabetes caused by pancreatic overexpression of insulin-like growth factor II (IGF-II/LDLR(-/-)ApoB(100/100) and LDLR(-/-)ApoB(100/100)) were studied. Distribution kinetics of choline analogue (18)F-fluoromethylcholine ((18)F-FMCH) was assessed in vivo by positron emission tomography (PET) imaging. Then, aortic uptakes of (18)F-FMCH and glucose analogue (18)F-fluorodeoxyglucose ((18)F-FDG), were assessed ex vivo by gamma counting and autoradiography of tissue sections. The (18)F-FMCH uptake in atherosclerotic plaques was further compared with macrophage infiltration and the plasma levels of cytokines and metabolic markers.
resultsThe aortas of all hypercholesterolemic mice showed large, macrophage-rich atherosclerotic plaques. The plaque burden and densities of macrophage subtypes were similar in diabetic and non-diabetic animals. The blood clearance of (18)F-FMCH was rapid. Both the absolute (18)F-FMCH uptake in the aorta and the aorta-to-blood uptake ratio were higher in diabetic than in non-diabetic mice. In autoradiography, the highest (18)F-FMCH uptake co-localized with macrophage-rich atherosclerotic plaques. (18)F-FMCH uptake in plaques correlated with levels of total cholesterol, insulin, C-peptide and leptin. In comparison with (18)F-FDG, (18)F-FMCH provided similar or higher plaque-to-background ratios in diabetic mice.
conclusionsType 2 diabetes enhances the uptake of choline that reflects inflammation in atherosclerotic plaques in mice. PET tracer (18)F-FMCH is a potential tool to study vascular inflammation associated with diabetes.
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Registered trials
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