ArticleParasitology research2016
PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.
Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Establishment and preliminary application of PCR-RFLP genotyping method for Giardia duodenalis in goats.BMC veterinary research · 2024Article
- A Multiplex PCR Assay for Simultaneous Detection ofVeterinary sciences · 2024Article
- Review
- Frequency and genotyping ofVeterinary research forum : an international quarterly journal · 2023Article
- Rapid on-site diagnosis of canine giardiosis: time versus performance.Parasites & vectors · 2020Article
- Development of TParasitology research · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia duodenalis is a zoonotic protozoan that parasitizes the upper small intestine of human and many mammals including dogs. To develop a restriction fragment length polymorphism (RFLP) method for typing zoonotic (A, B) and host-specific (C, D) assemblages of G. duodenalis from dog, β-giardin gene was amplified with design primer pairs B3 and B4. The PCR products were digested with restriction enzyme Afa I and Msp I; then, PCR-RFLP method was compared with HRM genotyping and sequencing method for G. duodenalis from dog. The results showed that each of assemblages A-D had unique restriction pattern, which was consistent with the predictive results. Among 21 samples tested by PCR-RFLP, 1 human-derived and 8 dog-derived G. duodenalis were identified as assemblage A; 5 dog-derived G. duodenalis as assemblage C; 7 dog-derived G. duodenalis as assemblage D, which were coincided with the HRM genotyping and sequencing results. It is concluded that the PCR-RFLP is quick, easy, and accurate method for the sequence typing of G. duodenalis zoonotic and specific assemblages from dogs.
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