Evidence map›Paper›PMID 26851580›Full record

ArticleBiosensors & bioelectronics2016

Electrical response of a B lymphoma cell line latently infected with Kaposi's sarcoma herpesvirus.

Mohammadali Safavieh, Sultan Khetani, Franceline Juillard, Vivasvat Kaul, Manoj Kumar Kanakasabapathy, Kenneth M Kaye, Hadi Shafiee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Study on non-bioparticles andRSC advances · 2020
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mohammadali SafaviehDivision of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Sultan KhetaniDivision of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Franceline JuillardDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Vivasvat KaulDivision of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Manoj Kumar KanakasabapathyDivision of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Kenneth M KayeDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Hadi ShafieeDivision of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: hshafiee@research.bwh.harvard.edu.

Funding

GENETIC AND BIOCHEMICAL STUDIES OF THE KSHV LANA GENER01CA082036 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI KAYE, KENNETH M · 1999 to 2019
$7.6M
KSHV Latent Infection ReplicationR01DE025208 · NIDCR · BRIGHAM AND WOMEN'S HOSPITAL · PI KAYE, KENNETH M · 2015 to 2025
$5.3M
Sensitive and quantitative point-of-care viral load testing on paperR01AI118502 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2016 to 2020
$2.2M
Label-free HIV-1 Viral Load Measurement through a Contactless Electrode MicrochipF32AI102590 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2013 to 2015
$171k
NCI NIH HHS NIH NCI CA082036NCI NIH HHS R01 CA082036NIAID NIH HHS 1R01AI118502NIAID NIH HHS F32 AI102590NIAID NIH HHS F32AI102590NIAID NIH HHS R01 AI118502NIDCR NIH HHS NIH NIDCR DE025208NIDCR NIH HHS R01 DE025208
6 · The paper itself

Abstract

Certain viruses, such as herpesviruses, are capable of persistent and latent infection of host cells. Distinguishing and separating live, latently infected cells from uninfected cells is not easily attainable using current approaches. The ability to perform such separation would greatly enhance the ability to study primary, infected cells and potentially enable elimination of latently infected cells from the host. Here, the dielectrophoretic response of B cells infected with Kaposi's sarcoma-associated herpesvirus (KSHV) were investigated and compared to uninfected B cells. We evaluated the effect of applied voltage, signal frequency, and flow rate of the sample on the cell capture efficiency. We achieved 37.1% ± 8.5% difference in capture efficiencies between latently KSHV-infected and uninfected BJAB B lymphoma cells at the chip operational conditions of 1V, 50 kHz and 0.02 μl/min sample flow rate. Our results show that latently infected B lymphoma cells demonstrated significantly different electrical response compared to uninfected B cells and DEP-based microchips can be potentially used for sorting latently infected cells based on their electrical properties.

Indexed as

Biosensing TechniquesHerpesvirus 8, HumanHumansLymphoma, B-CellSarcoma, KaposiViral ProteinsViral Proteins

Identifiers

PMID26851580
PMCPMC4779410

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.