Evidence map›Paper›PMID 26851521›Full record

ArticleJournal of immunological methods2016

Simple and reliable genotyping protocol for mouse Prkdc(SCID) mutation.

Rolen M Quadros, Larisa Y Poluektova, Channabasavaiah B Gurumurthy

Open access · greenAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Antiretroviral Drug Metabolism in Humanized PXR-CAR-CYP3A-NOG Mice.The Journal of pharmacology and experimental therapeutics · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rolen M QuadrosMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA.
Larisa Y PoluektovaDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Channabasavaiah B GurumurthyMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA; Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA.
University of Nebraska Medical Center · US

Funding

The Molecular Biology of Neurosurgery SystemsP20GM103471 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI SMITH, SHELLEY D · 2012 to 2013
$4.1M
Nebraska Center for VirologyP30GM103509 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 2012 to 2014
$3.3M
Center for Humanized MiceR24OD018546 · OD · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GORANTLA, SANTHI, POLUEKTOVA, LARISA Y · 2014 to 2017
$3.2M
SMART HANDR01AG043540 · NIA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GENDELMAN, HOWARD E, POLUEKTOVA, LARISA Y · 2013 to 2017
$3.1M
Humanized Mice as a Tool to Monitor HIV Brain Reservoirs and Effects of Substance AbuseR21DA041018 · NIDA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GORANTLA, SANTHI, POLUEKTOVA, LARISA Y · 2015 to 2017
$562k
NIA NIH HHS R01 AG043540NIDA NIH HHS R21 DA041018NIGMS NIH HHS P20 GM103471NIGMS NIH HHS P20GM103471NIGMS NIH HHS P30 GM103509NIH HHS NIH/1R24OD018546-01NIH HHS R24 OD018546
6 · The paper itself

Abstract

Mutant mouse models, genetically-engineered or spontaneous-mutations, serve as valuable tools for biomedical research. Genotyping of mutant mice is a critical requirement for maintaining the colony, to breed with other mutants and to match the phenotypic observations. The SCID (Severe Combine Immuno Deficiency) mouse model has been extensively used as a common background-strain in many immunology and transplantation studies. Many different types of assays, including Restriction Fragment Length Polymorphism (RFLP), confronting two primer pairs PCR and end-point methods have been attempted for establishing a genotyping protocol for the SCID mutation. However, the best method that is thought to be reliable is sequencing, which requires additional time and resources to perform on a routine basis. In this report, we describe a novel RFLP assay that is simple and reliable. The method is validated by sequencing analysis, and this novel method can be adapted for routine genotyping of SCID model.

Indexed as

AnimalsDisease Models, AnimalDNA-Activated Protein KinaseDNA-Binding ProteinsDNA Mutational AnalysisGenotyping TechniquesMiceMice, SCIDMutationNuclear ProteinsPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthDNA-Activated Protein KinaseDNA-Binding ProteinsNuclear ProteinsPrkdc protein, mouseGenotypingHumanized miceNSGRFLPSCID

Identifiers

PMID26851521
PMCPMC4818111
OpenAlexW2257092307

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.