ArticleNucleic acids research2016
Kissing-loop interaction between 5' and 3' ends of tick-borne Langat virus genome 'bridges the gap' between mosquito- and tick-borne flaviviruses in mechanisms of viral RNA cyclization: applications for virus attenuation and vaccine development.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Development and validation of a DNA-launched, intron-stabilized Langat virus infectious clone and reporter viruses.Virology · 2026Article
- Langat Virus Biology and Infection.Reviews in medical virology · 2026Review
- Breast milk transmission and involvement of mammary glands in tick-borne flavivirus infected mice.Journal of virology · 2024Article
- Examples of Structural Motifs in Viral Genomes and Approaches for RNA Structure Characterization.International journal of molecular sciences · 2022Review
- Structure and function of capsid protein in flavivirus infection and its applications in the development of vaccines and therapeutics.Veterinary research · 2021Review
- Epididymal epithelium propels early sexual transmission of Zika virus in the absence of interferon signaling.Nature communications · 2021Article
- Information Encoded by the Flavivirus Genomes beyond the Nucleotide Sequence.International journal of molecular sciences · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
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- Article
- Routes of Zika virus dissemination in the testis and epididymis of immunodeficient mice.Nature communications · 2018Article
- MicroRNA-Attenuated Virus Vaccines.Non-coding RNA · 2018Review
- Nanopore electric snapshots of an RNA tertiary folding pathway.Nature communications · 2017Article
- Article
- Article
- Functional Information Stored in the Conserved Structural RNA Domains of Flavivirus Genomes.Frontiers in microbiology · 2017Review
- Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization.eLife · 2016Article
- Concurrent micro-RNA mediated silencing of tick-borne flavivirus replication in tick vector and in the brain of vertebrate host.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Insertion of microRNA target sequences into the flavivirus genome results in selective tissue-specific attenuation and host-range restriction of live attenuated vaccine viruses. However, previous strategies for miRNA-targeting did not incorporate a mechanism to prevent target elimination under miRNA-mediated selective pressure, restricting their use in vaccine development. To overcome this limitation, we developed a new approach for miRNA-targeting of tick-borne flavivirus (Langat virus, LGTV) in the duplicated capsid gene region (DCGR). Genetic stability of viruses with DCGR was ensured by the presence of multiple cis-acting elements within the N-terminal capsid coding region, including the stem-loop structure (5'SL6) at the 3' end of the promoter. We found that the 5'SL6 functions as a structural scaffold for the conserved hexanucleotide motif at its tip and engages in a complementary interaction with the region present in the 3' NCR to enhance viral RNA replication. The resulting kissing-loop interaction, common in tick-borne flaviviruses, supports a single pair of cyclization elements (CYC) and functions as a homolog of the second pair of CYC that is present in the majority of mosquito-borne flaviviruses. Placing miRNA targets into the DCGR results in superior attenuation of LGTV in the CNS and does not interfere with development of protective immunity in immunized mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.