ArticleJournal of structural biology2016
Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins.
Article in Journal of structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
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- The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins.Physiological reports · 2025Article
- Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.Cell chemical biology · 2024Article
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- Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Glycosylated, Lipid-Binding, CDR-Like Domains of SARS-CoV-2 ORF8 Indicate Unique Sites of Immune Regulation.Microbiology spectrum · 2023Article
- In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy.Communications biology · 2023Article
- Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.Nature structural & molecular biology · 2022Article
- BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties.Molecular biotechnology · 2022Article
- A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.Communications medicine · 2021Article
- Cryo-EM structure of native human uromodulin, a zona pellucida module polymer.The EMBO journal · 2020Article
- Structure of the RECK CC domain, an evolutionary anomaly.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Analysis of Wild Type LbCpf1 Protein, and PAM Recognition Variants, in a Cellular Context.Frontiers in genetics · 2020Article
- Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.Nature communications · 2019Article
- Structure of mammalian plasma fetuin-B and its mechanism of selective metallopeptidase inhibition.IUCrJ · 2019Article
- Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification, as well as provide crystal-packing interactions for increased crystallizability. Using this cell type-independent approach, we could increase the expression of secreted and intracellular human proteins up to 200-fold. By molecular replacement with MBP, we readily determined five novel high-resolution structures of rigid fusions of targets that otherwise defied crystallization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.