ArticlePloS one2016
Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Therapeutic Effects of ProbioticFood science & nutrition · 2026Article
- Biological mechanisms of dopamine DFrontiers in endocrinology · 2025Article
- Development of Novel Tools for Dissection of Central Versus Peripheral Dopamine D2-Like Receptor Signaling in Dysglycemia.Diabetes · 2024Article
- Ultrasensitive Hierarchical AuNRs@SiOInternational journal of nanomedicine · 2024Article
- Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy.Cell calcium · 2023Article
- A pre-clinical study to investigate the anti-diabetic potential ofJournal of diabetes and metabolic disorders · 2023Article
- Nomogram model for the risk of insulin resistance in obese children and adolescents based on anthropomorphology and lipid derived indicators.BMC public health · 2023Article
- Roles of Pancreatic Islet Catecholamine Neurotransmitters in Glycemic Control and in Antipsychotic Drug-Induced Dysglycemia.Diabetes · 2023Review
- Development and validation of a new robust RP-HPLC method for simultaneous quantitation of insulin and pramlintide in non-invasive and smart glucose-responsive microparticles.Research in pharmaceutical sciences · 2022Article
- Dual pancreatic adrenergic and dopaminergic signaling as a therapeutic target of bromocriptine.iScience · 2022Article
- TG: HDL-C Ratio as Insulin Resistance Marker for Metabolic Syndrome in Children With Obesity.Frontiers in endocrinology · 2022Article
- Dopamine regulates pancreatic glucagon and insulin secretion via adrenergic and dopaminergic receptors.Translational psychiatry · 2021Article
- Personalized immunoglobulin aptamers for detection of multiple myeloma minimal residual disease in serum.Communications biology · 2020Article
- New roles for dopamine DMolecular psychiatry · 2020Article
- Dopamine DPsychoneuroendocrinology · 2020Article
- Dopamine DScientific reports · 2019Article
- Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.The Journal of biological chemistry · 2019Article
- Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion.Journal of visualized experiments : JoVE · 2018Article
- Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells.Journal of structural biology · 2018Article
- Probes for monitoring regulated exocytosis.Cell calcium · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.