Evidence map›Paper›PMID 26849429›Full record

ArticlePloS one2016

MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes.

Linyuan Shen, Lei Chen, Shunhua Zhang, Jingjing Du, Lin Bai, Yi Zhang, Yanzhi Jiang, Xuewei Li, Jinyong Wang, Li Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
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  8. Review
  9. The Role of MicroRNAs in Mitochondria-Mediated Eye Diseases.Frontiers in cell and developmental biology · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Linyuan ShenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lei ChenChongqing Academy of Animal Science, Rongchang, 402460, Chongqing, China.
Shunhua ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jingjing DuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lin BaiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yi ZhangDepartment of Animal Science, Xichang College, Xichang, 615000, Sichuan, China.
Yanzhi JiangDepartment of Biology, College of Life and Science, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xuewei LiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jinyong WangChongqing Academy of Animal Science, Rongchang, 402460, Chongqing, China.
Li ZhuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Sichuan Agricultural University · CNChongqing Academy of Animal Science · CNXichang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that affect the post-transcriptional regulation of various biological pathways. To date, it is not fully understood how miRNAs regulate mitochondrial biogenesis. This study aimed at the identification of the role of miRNA-27b in mitochondria biogenesis. The mitochondria content in C2C12 cells was significantly increased during myogenic differentiation and accompanied by a marked decrease of miRNA-27b expression. Furthermore, the expression of the predicted target gene of miRNA-27b, forkhead box j3 (Foxj3), was also increased during myogenic differentiation. Luciferase activity assays confirmed that miRNA-27b directly targets the 3'-untranslated region (3'-UTR) of Foxj3. Overexpression of miRNA-27b provoked a decrease of mitochondria content and diminished expression of related mitochondrial genes and Foxj3 both at mRNA and protein levels. The expression levels of downstream genes of Foxj3, such as Mef2c, PGC1α, NRF1 and mtTFA, were also decreased in C2C12 cells upon overexpression of miRNA-27b. These results suggested that miRNA-27b may affect mitochondria biogenesis by down-regulation of Foxj3 during myocyte differentiation.

Indexed as

MitochondriaOrganelle BiogenesisAnimalsCell DifferentiationCells, CulturedDNA-Binding ProteinsForkhead Transcription FactorsGene Expression RegulationMEF2 Transcription FactorsMiceMicroRNAsMuscle CellsNuclear Respiratory Factor 1Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaTranscription FactorsDNA-Binding ProteinsForkhead Transcription FactorsFoxJ3 protein, mouseMef2c protein, mouseMEF2 Transcription FactorsMicroRNAsMirn27 microRNA, mouseNrf1 protein, mouseNuclear Respiratory Factor 1Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPpargc1a protein, mouseTranscription Factors

Identifiers

PMID26849429
PMCPMC4746119
OpenAlexW2261072490

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.