ArticlePloS one2016
Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- Laccase-Catalyzed Biotransformation of Dihydrostilbenes and Phenanthrenes: A Route to Novel Natural Product Derivatives.ACS omega · 2026Article
- Improving the Properties of Laccase Through Heterologous Expression and Protein Engineering.Microorganisms · 2025Review
- Leveraging bacterial laccases to facilitate the decomposition of xenobiotic compounds: a review.3 Biotech · 2024Review
- The Contribution ofFoods (Basel, Switzerland) · 2024Article
- Recent advances in fungal xenobiotic metabolism: enzymes and applications.World journal of microbiology & biotechnology · 2023Review
- Article
- Fungal bioproducts for petroleum hydrocarbons and toxic metals remediation: recent advances and emerging technologies.Bioprocess and biosystems engineering · 2023Review
- Enhancing the production of a heterologous Trametes laccase (LacA) by replacement of the major cellulase CBH1 in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- Endocrine Disrupting Compounds (Nonylphenol and Bisphenol A)-Sources, Harmfulness and Laccase-Assisted Degradation in the Aquatic Environment.Microorganisms · 2022Review
- Circadian oscillations ineLife · 2022Article
- Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatment.AMB Express · 2022Article
- Effects onPlants (Basel, Switzerland) · 2021Article
- Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter.Microbial cell factories · 2020Article
- Laccases: structure, function, and potential application in water bioremediation.Microbial cell factories · 2019Review
- Article
- Article
- Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification.International journal of molecular sciences · 2018Review
- Synergetic integration of laccase and versatile peroxidase with magnetic silica microspheres towards remediation of biorefinery wastewater.Environmental science and pollution research international · 2017Article
- Overview on the Biochemical Potential of Filamentous Fungi to Degrade Pharmaceutical Compounds.Frontiers in microbiology · 2017Review
- Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal laccases are enzymes that have been studied because of their ability to decolorize and detoxify effluents; they are also used in paper bleaching, synthesis of polymers, bioremediation, etc. In this work we were able to express a laccase from Trametes (Pycnoporus) sanguineus in the filamentous fungus Trichoderma atroviride. For this purpose, a transformation vector was designed to integrate the gene of interest in an intergenic locus near the blu17 terminator region. Although monosporic selection was still necessary, stable integration at the desired locus was achieved. The native signal peptide from T. sanguineus laccase was successful to secrete the recombinant protein into the culture medium. The purified, heterologously expressed laccase maintained similar properties to those observed in the native enzyme (Km and kcat and kcat/km values for ABTS, thermostability, substrate range, pH optimum, etc). To determine the bioremediation potential of this modified strain, the laccase-overexpressing Trichoderma strain was used to remove xenobiotic compounds. Phenolic compounds present in industrial wastewater and bisphenol A (an endocrine disruptor) from the culture medium were more efficiently removed by this modified strain than with the wild type. In addition, the heterologously expressed laccase was able to decolorize different dyes as well as remove benzo[α]pyrene and phenanthrene in vitro, showing its potential for xenobiotic compound degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.