ArticlePloS one2016
High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 154 citations in OpenAlex.
- Evaluation of an AI-Assisted Colony Counting System Across Multiple Culture Media Using Standardized Pure Culture Plates.Microorganisms · 2026Article
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- Comparative evaluation of YOLOv8, YOLOv11, and RT-DETR for automated microcarrier colonization assessment in bioreactor cell cultures.Frontiers in bioengineering and biotechnology · 2026Article
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- Automated Image-Based Profiling of Pluripotent Stem Cell Colonies.bioRxiv : the preprint server for biology · 2025Article
- Colony-YOLO: A Lightweight Micro-Colony Detection Network Based on Improved YOLOv8n.Microorganisms · 2025Article
- C-COUNT: a convolutional neural network-based tool for automated scoring of erythroid colonies.Experimental hematology · 2025Article
- Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.Nature genetics · 2025Article
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- Erioflorin and Erioflorin Acetate Induce Cell Death in Advanced Prostate Cancer Through ROS Increase and NF-κB Inhibition.Journal of xenobiotics · 2025Article
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- The involvement of Elf5 in regulating keratinocyte proliferation and differentiation processes in skin.PloS one · 2025Article
- Quality Differences in Frozen Mackerel According to Thawing Method: Potential Classification via Hyperspectral Imaging.Foods (Basel, Switzerland) · 2024Article
- [PSI]-CIC: A Deep-Learning Pipeline for the Annotation of Sectored Saccharomyces cerevisiae Colonies.Bulletin of mathematical biology · 2024Article
- Prediction and Visualization of Total Volatile Basic Nitrogen in Yellow Croaker (Foods (Basel, Switzerland) · 2024Article
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- HERC5 downregulation in non-small cell lung cancer is associated with altered energy metabolism and metastasis.Journal of experimental & clinical cancer research : CR · 2024Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Counting cells and colonies is an integral part of high-throughput screens and quantitative cellular assays. Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies from digital images. For this purpose, I developed an ImageJ macro Cell Colony Edge and a CellProfiler Pipeline Cell Colony Counting, and compared them to other open-source digital methods and manual counts. The ImageJ macro Cell Colony Edge is valuable in counting cells and colonies, and measuring their area, volume, morphology, and intensity. In this study, I demonstrate that Cell Colony Edge is superior to other open-source methods, in speed, accuracy and applicability to diverse cellular assays. It can fulfill the need to automate colony/cell counting in high-throughput screens, colony forming assays, and cellular assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.