ArticleOncotarget2016
Identification of cis- and trans-acting elements regulating calretinin expression in mesothelioma cells.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The trial behind it
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Pleural effusion metastatic papillary thyroid carcinoma with dedifferentiation: a case report and review of the literature.Frontiers in oncology · 2025Article
- Serum Calretinin and Genetic Variability as a Prognostic and Predictive Factor in Malignant Mesothelioma.International journal of molecular sciences · 2023Article
- Article
- Rabies virus infection is associated with variations in calbindin D-28K and calretinin mRNA expression levels in mouse brain tissue.Archives of virology · 2023Article
- Viral Mimicry Response Is Associated With Clinical Outcome in Pleural Mesothelioma.JTO clinical and research reports · 2022Article
- Characterization and Localization ofAnimals : an open access journal from MDPI · 2020Article
- Cytosolic CaCold Spring Harbor perspectives in biology · 2020Review
- Calretinin promotes invasiveness and EMT in malignant mesothelioma cells involving the activation of the FAK signaling pathway.Oncotarget · 2018Article
- Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter.BMC cancer · 2018Article
- Absence of calretinin protein expression in malignant mesotheliomas from asbestos-exposed NF2Biomarker research · 2018Article
- Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression.Frontiers in oncology · 2018Article
- Posttranscriptional Regulation Controls Calretinin Expression in Malignant Pleural Mesothelioma.Frontiers in genetics · 2017Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Calretinin (CALB2) is a diagnostic marker for epithelioid mesothelioma. It is also a prognostic marker since patients with tumors expressing high calretinin levels have better overall survival. Silencing of calretinin decreases viability of epithelioid mesothelioma cells. Our aim was to elucidate mechanisms regulating calretinin expression in mesothelioma. Analysis of calretinin transcript and protein suggested a control at the mRNA level. Treatment with 5-aza-2'-deoxycytidine and analysis of TCGA data indicated that promoter methylation is not likely to be involved. Therefore, we investigated CALB2 promoter by analyzing ~1kb of genomic sequence surrounding the transcription start site (TSS) + 1 using promoter reporter assay. Deletion analysis of CALB2 proximal promoter showed that sequence spanning the -161/+80bp region sustained transcriptional activity. Site-directed analysis identified important cis-regulatory elements within this -161/+80bp CALB2 promoter. EMSA and ChIP assays confirmed binding of NRF-1 and E2F2 to the CALB2 promoter and siRNA knockdown of NRF-1 led to decreased expression of calretinin. Cell synchronization experiment showed that calretinin expression was cell cycle regulated with a peak of expression at G1/S phase. This study provides the first insight in the regulation of CALB2 expression in mesothelioma cells.
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Registered trials
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