ArticleGenome biology2016
Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay.
Article in Genome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics.Nature medicine · 2021Pooled it
- ECHOS enables spatial epigenome profiling at subcellular resolution.bioRxiv : the preprint server for biology · 2026Article
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Functional, transcriptomic, and lipidomic studies of theMicrobiology spectrum · 2024Article
- Uniform quantification of single-nucleus ATAC-seq data with Paired-Insertion Counting (PIC) and a model-based insertion rate estimator.Nature methods · 2024Article
- Functional annotation of the animal genomes: An integrated annotation resource for the horse.PLoS genetics · 2023Review
- Intrinsic bias estimation for improved analysis of bulk and single-cell chromatin accessibility profiles using SELMA.Nature communications · 2022Article
- Extensive evaluation of ATAC-seq protocols for native or formaldehyde-fixed nuclei.BMC genomics · 2022Article
- Profiling chromatin accessibility in formalin-fixed paraffin-embedded samples.Genome research · 2022Article
- Comparative methylomics and chromatin accessibility analysis inHorticulture research · 2022Article
- High-throughput single-cell epigenomic profiling by targeted insertion of promoters (TIP-seq).The Journal of cell biology · 2021Article
- FACT-seq: profiling histone modifications in formalin-fixed paraffin-embedded samples with low cell numbers.Nucleic acids research · 2021Article
- High-content single-cell combinatorial indexing.Nature biotechnology · 2021Article
- Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2).Nature protocols · 2021Article
- Decoding the Equine Genome: Lessons from ENCODE.Genes · 2021Review
- Tagmentation-based single-cell genomics.Genome research · 2021Review
- Review
- Chromatin accessibility profiling methods.Nature reviews. Methods primers · 2021Article
- Tn5 Transposase Applied in Genomics Research.International journal of molecular sciences · 2020Review
- Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Chromatin accessibility captures in vivo protein-chromosome binding status, and is considered an informative proxy for protein-DNA interactions. DNase I and Tn5 transposase assays require thousands to millions of fresh cells for comprehensive chromatin mapping. Applying Tn5 tagmentation to hundreds of cells results in sparse chromatin maps. We present a transposome hypersensitive sites sequencing assay for highly sensitive characterization of chromatin accessibility. Linear amplification of accessible DNA ends with in vitro transcription, coupled with an engineered Tn5 super-mutant, demonstrates improved sensitivity on limited input materials, and accessibility of small regions near distal enhancers, compared with ATAC-seq.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.