ArticleNature protocols2016
Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 97 citations in OpenAlex.
- Modeling diffuse midline glioma through triple-electrode in utero electroporation of the developing mouse pons.PloS one · 2026Article
- Bidirectional interaction between protocadherin 8 and the transcription factor Dbx1 regulates cerebral cortex development.Development (Cambridge, England) · 2026Article
- Prenatal Downregulation of CBThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Developmental transformations of Purkinje cells tracked by DNA electrokinetic mobility.Cell reports methods · 2025Article
- Alterations in dopaminergic innervation and receptors in focal cortical dysplasia.Brain : a journal of neurology · 2025Article
- Dual role of FOXG1 in regulating gliogenesis in the developing neocortex via the FGF signalling pathway.eLife · 2025Article
- Electroporation Techniques to Target Subependymal Zone Neurogenic Niche.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Distinct spatial distribution of potentiated dendritic spines in encoding- and recall-activated hippocampal neurons.Frontiers in molecular neuroscience · 2025Article
- Sparse Labeling, Rapid Clearing, and Native Fluorescence Light Sheet Imaging in the Developing Rodent Cerebellum.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Knockdown of Lhx6 during embryonic development results in neurophysiological alterations and behavioral deficits analogous to schizophrenia in adult rats.Schizophrenia research · 2024Article
- Anti-seizure gene therapy for focal cortical dysplasia.Brain : a journal of neurology · 2024Article
- A developmental increase of inhibition promotes the emergence of hippocampal ripples.Nature communications · 2024Article
- Daily rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability.Nature communications · 2023Article
- Enhancing Precision and Efficiency of Cas9-Mediated Knockin Through Combinatorial Fusions of DNA Repair Proteins.The CRISPR journal · 2023Article
- Advances in cellular resolution microscopy for brain imaging in rats.Neurophotonics · 2023Review
- Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders.International journal of molecular sciences · 2023Review
- Targeted approaches to delineate neuronal morphology during early development.Frontiers in cellular neuroscience · 2023Review
- Circuit-selective cell-autonomous regulation of inhibition in pyramidal neurons by Ste20-like kinase.Cell reports · 2022Article
- Loss of non-motor kinesin KIF26A causes congenital brain malformations via dysregulated neuronal migration and axonal growth as well as apoptosis.Developmental cell · 2022Article
- Aβ42 oligomers trigger synaptic loss through CAMKK2-AMPK-dependent effectors coordinating mitochondrial fission and mitophagy.Nature communications · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol is an extension to:Nat. Protoc. 1, 1552-1558 (2006); doi:10.1038/nprot.2006.276; published online 9 November 2006This article describes how to reliably electroporate with DNA plasmids rodent neuronal progenitors of the hippocampus; the motor, prefrontal and visual cortices; and the cerebellum in utero. As a Protocol Extension article, this article describes an adaptation of an existing Protocol and offers additional applications. The earlier protocol describes how to electroporate mouse embryos using two standard forceps-type electrodes. In the present protocol, additional electroporation configurations are possible because of the addition of a third electrode alongside the two standard forceps-type electrodes. By adjusting the position and polarity of the three electrodes, the electric field can be directed with great accuracy to different neurogenic areas. Bilateral transfection of brain hemispheres can be achieved after a single electroporation episode. Approximately 75% of electroporated embryos survive to postnatal ages, and depending on the target area, 50-90% express the electroporated vector. The electroporation procedure takes 1 h 35 min. The protocol is suitable for the preparation of animals for various applications, including histochemistry, behavioral studies, electrophysiology and in vivo imaging.
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Registered trials
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