Evidence map›Paper›PMID 26843527›Full record

ArticleRNA (New York, N.Y.)2016

The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases.

Domagoj Hodko, Taylor Ward, Guillaume Chanfreau

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Human REXO4 is Required for Cell Cycle Progression.bioRxiv : the preprint server for biology · 2025
    Article
  2. Article
  3. Article
  4. Biogenesis of RNA Polymerases in Yeast.Frontiers in molecular biosciences · 2021
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Domagoj HodkoDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA.
Taylor WardDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA.
Guillaume ChanfreauDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA.
University of California, Los Angeles · US

Funding

THE CONTROL OF GENE EXPRESSION BY EUKARYOTIC RNASE IIIR01GM061518 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHANFREAU, GUILLAUME F · 2000 to 2017
$5.5M
NIGMS NIH HHS GM61518NIGMS NIH HHS R01 GM061518
6 · The paper itself

Abstract

Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3'UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5'-3' DExD/H-box RNA helicase Dhh1p and the 3'-5' exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases.

Indexed as

Gene Expression Regulation, Fungal3' Untranslated RegionsBase SequenceExoribonucleasesRNA, MessengerRNA StabilitySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsTranscription, Genetic3' Untranslated RegionsExoribonucleasesRex2 protein, S cerevisiaeRex3 protein, S cerevisiaeRNA, MessengerRtr1 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsautoregulationdegradationREXRNARTR1yeast

Identifiers

PMID26843527
PMCPMC4793211
OpenAlexW2267526330

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.