ArticleMedical and veterinary entomology2016
Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies.
Article in Medical and veterinary entomology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- House Flies Are Underappreciated Yet Important Reservoirs and Vectors of Microbial Threats to Animal and Human Health.Microorganisms · 2023Review
- Flies as Vectors and Potential Sentinels for Bacterial Pathogens and Antimicrobial Resistance: A Review.Veterinary sciences · 2022Review
- Integrated Metabolomics and Network Pharmacology Study on Immunoregulation Mechanisms ofEvidence-based complementary and alternative medicine : eCAM · 2019Article
- Dose-Dependent Effects on Replication and Persistence of Salmonella enterica serovar Typhimurium in House Flies (Diptera: Muscidae).Journal of medical entomology · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The adult house fly Musca domestica (L.) (Diptera: Muscidae) can disseminate bacteria from microbe-rich substrates to areas in which humans and domesticated animals reside. Because bacterial abundance fluctuates widely across substrates, flies encounter and ingest varying amounts of bacteria. This study investigated the dose-dependent survival of bacteria in house flies. Flies were fed four different 'doses' of green fluorescent protein (GFP)-expressing Escherichia coli (GFP E. coli) (very low, low, medium, high) and survival was determined at 1, 4, 10 and 22 h post-ingestion by culture and epifluorescent microscopy. Over 22 h, the decline in GFP E. coli was significant in all treatments (P < 0.04) except the very low dose treatment (P = 0.235). Change in survival (ΔS) did not differ between flies fed low and very low doses of bacteria across all time-points, although ΔS in both treatments differed from that in flies fed high and medium doses of bacteria at several time-points. At 4, 10 and 22 h, GFP E. coli ΔS significantly differed between medium and high dose-fed flies. A threshold dose, above which bacteria are detected and destroyed by house flies, may exist and is likely to be immune-mediated. Understanding dose-dependent bacterial survival in flies can help in predicting bacteria transmission potential.
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