ArticleNature communications2016
Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 114 citations in OpenAlex.
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- Real-time nitric oxide detection in cytokine stimulated cancer cells and macrophages.Nitric oxide : biology and chemistry · 2025Article
- Spatiotemporal Nitric Oxide Modulation via Electrochemical Platform to Profile Tumor Cell Response.Angewandte Chemie (International ed. in English) · 2024Article
- Ratiometric optical dual gas sensor for simultaneous detection of nitric oxide and oxygen intended for healthcare, biological, and biomedical applications.RSC advances · 2024Article
- Nitric Oxide Signaling and Sensing in Age-Related Diseases.Antioxidants (Basel, Switzerland) · 2024Review
- Astrocytes produce nitric oxide via nitrite reduction in mitochondria to regulate cerebral blood flow during brain hypoxia.Cell reports · 2023Article
- Volatile anesthetics inhibit presynaptic cGMP signaling to depress presynaptic excitability in rat hippocampal neurons.Neuropharmacology · 2023Article
- Manipulating D-A interaction to achieve stable photoinduced organic radicals in triphenylphosphine crystals.Chemical science · 2023Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
- Nitric Oxide Sensing by a Blue Fluorescent Protein.Antioxidants (Basel, Switzerland) · 2022Article
- Probing Subcellular Iron Availability with Genetically Encoded Nitric Oxide Biosensors.Biosensors · 2022Article
- Expression of CD70 Modulates Nitric Oxide and Redox Status in Endothelial Cells.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels.Redox biology · 2022Article
- A two-step screening to optimize the signal response of an auto-fluorescent protein-based biosensor.RSC advances · 2022Article
- Complexities of the chemogenetic toolkit: Differential mDAAO activation by d-amino substrates and subcellular targeting.Free radical biology & medicine · 2021Article
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
Abstract
Nitric oxide () is a free radical with a wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development of novel multicoloured fluorescent quenching-based probes by fusing a bacteria-derived -binding domain close to distinct fluorescent protein variants. These genetically encoded probes, referred to as geNOps, provide a selective, specific and real-time read-out of cellular dynamics and, hence, open a new era of bioimaging. The combination of geNOps with a Ca(2+) sensor allowed us to visualize and Ca(2+) signals simultaneously in single endothelial cells. Moreover, targeting of the probes was used to detect signals within mitochondria. The geNOps are useful new tools to further investigate and understand the complex patterns of signalling on the single (sub)cellular level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.