ArticleProceedings of the National Academy of Sciences of the United States of America2016
Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Discovery of the rosalexin pathway expands the modular network of maize diterpenoid phytoalexins.Research square · 2026Article
- Analysis genome of Pseudotaxus chienii reveals insights into the origin and evolution of taxane biosynthesis.Nature communications · 2025Article
- Protein design drives synthetic biology research of plant natural products.Biodesign research · 2025Review
- Noncanonical terpene cyclases for the biosynthesis of diterpenoids regulating chlamydospore formation in plant-associated Trichoderma.Nature communications · 2025Article
- On the Role of Hydrogen Migrations in the Taxadiene System.Angewandte Chemie (International ed. in English) · 2025Article
- Engineering terpene synthases and their substrates for the biocatalytic production of terpene natural products and analogues.Chemical communications (Cambridge, England) · 2025Review
- Unravelling the aromatic symphony: redirecting bifunctional mushroom synthases towards linalool monofunctionality.Advanced biotechnology · 2025Article
- Fine-Tuning the Function of Farnesene Synthases for Selective Synthesis of Farnesene Stereoisomers.Journal of agricultural and food chemistry · 2024Article
- Plants against cancer: towards green Taxol production through pathway discovery and metabolic engineering.aBIOTECH · 2024Review
- A diterpene synthase from the sandflyProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Decoding Catalysis by Terpene Synthases.ACS catalysis · 2023Review
- Structural Understanding of Fungal Terpene Synthases for the Formation of Linear or Cyclic Terpene Products.ACS catalysis · 2023Article
- Biotechnological potential and initial characterization of two novel sesquiterpene synthases from Basidiomycota Coniophora puteana for heterologous production of δ-cadinol.Microbial cell factories · 2022Article
- Molecular Determinants of Carbocation Cyclisation in Bacterial Monoterpene Synthases.Chembiochem : a European journal of chemical biology · 2022Article
- Understanding the competing pathways leading to hydropyrene and isoelisabethatriene.Beilstein journal of organic chemistry · 2022Article
- Mechanistic Insights into the Formation of the 6,10-Bicyclic Eunicellane Skeleton by the Bacterial Diterpene Synthase Bnd4.Angewandte Chemie (International ed. in English) · 2021Article
- An extremely promiscuous terpenoid synthase from the Lamiaceae plantPlant communications · 2021Article
- Mining methods and typical structural mechanisms of terpene cyclases.Bioresources and bioprocessing · 2021Review
- Recent Advances in Metabolic Engineering, Protein Engineering, and Transcriptome-Guided Insights Toward Synthetic Production of Taxol.Frontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Class I terpene synthases generate the structural core of bioactive terpenoids. Deciphering structure-function relationships in the reactive closed complex and targeted engineering is hampered by highly dynamic carbocation rearrangements during catalysis. Available crystal structures, however, represent the open, catalytically inactive form or harbor nonproductive substrate analogs. Here, we present a catalytically relevant, closed conformation of taxadiene synthase (TXS), the model class I terpene synthase, which simulates the initial catalytic time point. In silico modeling of subsequent catalytic steps allowed unprecedented insights into the dynamic reaction cascades and promiscuity mechanisms of class I terpene synthases. This generally applicable methodology enables the active-site localization of carbocations and demonstrates the presence of an active-site base motif and its dominating role during catalysis. It additionally allowed in silico-designed targeted protein engineering that unlocked the path to alternate monocyclic and bicyclic synthons representing the basis of a myriad of bioactive terpenoids.
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