ArticleProceedings of the National Academy of Sciences of the United States of America2016
In vivo imaging reveals an essential role of vasoconstriction in rupture of the ovarian follicle at ovulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 51 citations in OpenAlex.
- Obesity disrupts ovarian hemodynamics during the preovulatory and luteal phases in mice†.Biology of reproduction · 2026Article
- Article
- Microscale pressure measurements and optical coherence tomography reveal time-dependent biomechanical stages of ovulation in mice.iScience · 2025Article
- Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice.Journal of visualized experiments : JoVE · 2025Article
- CCAAT/Enhancer-Binding Proteins α and β Regulate Ovulation and Gene Expression via Dose- and Stage-Dependent Mechanisms.Endocrinology · 2025Article
- Neurotensin Regulates Primate Ovulation Via Multiple Neurotensin Receptors.Endocrinology · 2025Article
- Luteinizing hormone-induced changes in the structure of mammalian preovulatory follicles.Current topics in developmental biology · 2025Review
- Ex vivo imaging reveals the spatiotemporal control of ovulation.Nature cell biology · 2024Article
- Follicle-intrinsic and spatially distinct molecular programs drive follicle rupture and luteinization during ex vivo mammalian ovulation.Communications biology · 2024Article
- Novel imaging and biophysical approaches to study tissue hydraulics in mammalian folliculogenesis.Biophysical reviews · 2024Review
- Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization.Bioengineering (Basel, Switzerland) · 2024Article
- Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle†.Biology of reproduction · 2024Article
- A spatiotemporal molecular atlas of the ovulating mouse ovary.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Lymphatic vasculature in ovarian cancer.Biochimica et biophysica acta. Reviews on cancer · 2023Review
- The role of zinc in follicular development.Molecular biology reports · 2023Review
- Dynamic regulation of semaphorin 7A and adhesion receptors in ovarian follicle remodeling and ovulation.Frontiers in cell and developmental biology · 2023Article
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- Nuclear Progestin Receptor-mediated Linkage of Blood Coagulation and Ovulation.Endocrinology · 2022Article
- Intraovarian, Isoform-Specific Transcriptional Roles of Progesterone Receptor in Ovulation.Cells · 2022Article
- Endothelin 2: a key player in ovulation and fertility.Reproduction (Cambridge, England) · 2022Review
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
Rupture of the ovarian follicle releases the oocyte at ovulation, a timed event that is critical for fertilization. It is not understood how the protease activity required for rupture is directed with precise timing and localization to the outer surface, or apex, of the follicle. We hypothesized that vasoconstriction at the apex is essential for rupture. The diameter and blood flow of individual vessels and the thickness of the apical follicle wall were examined over time to expected ovulation using intravital multiphoton microscopy. Vasoconstriction of apical vessels occurred within hours preceding follicle rupture in wild-type mice, but vasoconstriction and rupture were absent in Amhr2(cre/+)SmoM2 mice in which follicle vessels lack the normal association with vascular smooth muscle. Vasoconstriction is not simply a response to reduced thickness of the follicle wall; vasoconstriction persisted in wild-type mice when thinning of the follicle wall was prevented by infusion of protease inhibitors into the ovarian bursa. Ovulation was inhibited by preventing the periovulatory rise in the expression of the vasoconstrictor endothelin 2 by follicle cells of wild-type mice. In these mice, infusion of vasoconstrictors (either endothelin 2 or angiotensin 2) into the bursa restored the vasoconstriction of apical vessels and ovulation. Additionally, infusion of endothelin receptor antagonists into the bursa of wild-type mice prevented vasoconstriction and follicle rupture. Processing tissue to allow imaging at increased depth through the follicle and transabdominal ultrasonography in vivo showed that decreased blood flow is restricted to the apex. These results demonstrate that vasoconstriction at the apex of the follicle is essential for ovulation.
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