ArticleScientific reports2016
Depletion of the LINC complex disrupts cytoskeleton dynamics and meiotic resumption in mouse oocytes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Nucleoskeletal Proteins in Early Embryogenesis.Advances in experimental medicine and biology · 2026Review
- Absence of CDK12 in oocyte leads to female infertility.Cell death & disease · 2025Article
- A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis inScience advances · 2023Article
- Homozygous missense mutation in CCDC155 disrupts the transmembrane distribution of CCDC155 and SUN1, resulting in non-obstructive azoospermia and premature ovarian insufficiency in humans.Human genetics · 2022Article
- Molecular mimicry between SARS-CoV-2 and the female reproductive system.American journal of reproductive immunology (New York, N.Y. : 1989) · 2021Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Diverse roles for CDK-associated activity during spermatogenesis.FEBS letters · 2019Review
- Molecular-Level Interactions between Engineered Materials and Cells.International journal of molecular sciences · 2019Review
- Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia.Cell reports · 2019Article
- Mechano-adaptation of the stem cell nucleus.Nucleus (Austin, Tex.) · 2018Review
- Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments.Scientific reports · 2017Article
- Function and regulation mechanism of Chk1 during meiotic maturation in porcine oocytes.Cell cycle (Georgetown, Tex.) · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SUN (Sad-1/UNC-84) and KASH (Klarsicht/ANC-1/Syne/homology) proteins constitute the linker of nucleoskeleton and cytoskeleton (LINC) complex on the nuclear envelope. To date, the SUN1/KASH5 complex is known to function as meiotic-specific factors. In this study, gene-silencing methods were used to explore the roles of SUN1 and KASH5 in mouse oocytes after prophase. SUN1 was detected throughout the nucleus; however, KASH5 was dispersed through the cell. After germinal vesicle breakdown (GVBD), SUN1 and KASH5 migrated during spindle formation and localized to the spindle poles at the MII stage. Most oocytes were arrested at the germinal vesicle (GV) stage after depletion of either SUN1 or KASH5. The DNA damage response was triggered in SUN1-depleted oocytes and thus gave rise to the G2/M checkpoint protein, p-CHK1. Oocytes that underwent GVBD had relatively small and abnormal spindles and lower levels of cytoplasm F-actin mesh. Immunofluorescence results also indicated the dislocation of pericentrin and P150(Glued) after SUN1 or KASH5 depletion. Furthermore, KASH5 localized exclusively near the oocyte cortex after SUN1 depletion, but SUN1 localization was unaffected in KASH5-depleted oocytes. Taken together, the results suggest that SUN1 and KASH5 are essential factors in the regulation of meiotic resumption and spindle formation.
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