ArticleProteomics2016
Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays.
Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Quantum Dot Bioconjugates Enable Order(s)-of-Magnitude Enhancement of Immunoglobulin E Detection Sensitivity.ACS omega · 2026Article
- Functionalized Quantum Dot-Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva.Journal of biophotonics · 2026Article
- Risk factors for SARS-CoV-2 seropositivity in a health care worker population during the early pandemic.BMC infectious diseases · 2023Article
- Characterization of pathogen-inactivated COVID-19 convalescent plasma and responses in transfused patients.Transfusion · 2022Article
- Predicting COVID-19 Severity with a Specific Nucleocapsid Antibody plus Disease Risk Factor Score.mSphere · 2021Article
- Distinct SARS-CoV-2 antibody reactivity patterns in coronavirus convalescent plasma revealed by a coronavirus antigen microarray.Scientific reports · 2021Article
- Estimated seroprevalence of SARS-CoV-2 antibodies among adults in Orange County, California.Scientific reports · 2021Article
- Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray.Nature communications · 2021Article
- A modular microarray imaging system for highly specific COVID-19 antibody testing.Lab on a chip · 2020Article
- A Modular Microarray Imaging System for Highly Specific COVID-19 Antibody Testing.bioRxiv : the preprint server for biology · 2020Article
- Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes.Journal of visualized experiments : JoVE · 2019Article
- Distinct Antibody Signatures Associated with Different Malaria Transmission Intensities in Zambia and Zimbabwe.mSphere · 2019Article
- Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific Antibodies.mSphere · 2018Article
- Article
- Challenges in paper-based fluorogenic optical sensing with smartphones.Nano convergence · 2018Review
- Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children.PLoS neglected tropical diseases · 2017Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Organic fluorescent dyes are widely used for the visualization of bound antibody in a variety of immunofluorescence assays. However, the detection equipment is often expensive, fragile, and hard to deploy widely. Quantum dots (Qdot) are nanocrystals made of semiconductor materials that emit light at different wavelengths according to the size of the crystal, with increased brightness and stability. Here, we have evaluated a small benchtop "personal" optical imager (ArrayCAM) developed for quantification of protein arrays probed by Qdot-based indirect immunofluorescence. The aim was to determine if the Qdot imager system provides equivalent data to the conventional organic dye-labeled antibody/laser scanner system. To do this, duplicate proteome microarrays of Vaccinia virus, Brucella melitensis and Plasmodium falciparum were probed with identical samples of immune sera, and IgG, IgA, and IgM profiles visualized using biotinylated secondary antibodies followed by a tertiary reagent of streptavidin coupled to either P3 (an organic cyanine dye typically used for microarrays) or Q800 (Qdot). The data show excellent correlation for all samples tested (R > 0.8) with no significant change of antibody reactivity profiles. We conclude that Qdot detection provides data equivalent to that obtained using conventional organic dye detection. The portable imager offers an economical, more robust, and deployable alternative to conventional laser array scanners.
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