Evidence map›Paper›PMID 26841926›Full record

ArticleBMC cancer2016

Associations of microbiota and toll-like receptor signaling pathway in esophageal adenocarcinoma.

Ali H Zaidi, Lori A Kelly, Rachael E Kreft, Mark Barlek, Ashten N Omstead, Daisuke Matsui, Natalie H Boyd, Kathryn E Gazarik, Megan I Heit, Laura Nistico and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 96 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Ali H ZaidiEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. azaidi@wpahs.org.
Lori A KellyEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. lkelly7@wpahs.org.
Rachael E KreftCenter of Excellence in Biofilm Research, Allegheny Health Network, Pittsburgh, PA, USA. rkreft1@wpahs.org.
Mark BarlekEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. mbarlek@gmail.com.
Ashten N OmsteadEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. aomstead@wpahs.org.
Daisuke MatsuiEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. dmatsui@wpahs.org.
Natalie H BoydEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. nboyd@wpahs.org.
Kathryn E GazarikEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. kgazarik@wpahs.org.
Megan I HeitEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. mheit1@wpahs.org.
Laura NisticoCenter of Excellence in Biofilm Research, Allegheny Health Network, Pittsburgh, PA, USA. lnistico@wpahs.org.
Pashtoon M KasiDepartment of Medicine, Mayo Clinic, Rochester, Minnesota, USA. pashtoon.kasi@gmail.com.
Tracy L SpirkCenter of Excellence in Biofilm Research, Allegheny Health Network, Pittsburgh, PA, USA. tspirk@wpahs.org.
Barbara ByersCenter of Excellence in Biofilm Research, Allegheny Health Network, Pittsburgh, PA, USA. bbyers1@wpahs.org.
Emily J LloydEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. elloyd1@wpahs.org.
Rodney J LandreneauEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. rlandren@wpahs.org.
Blair A JobeEsophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA, 15224, USA. bjobe1@wpahs.org.
Lung Institute · USAllegheny Health Network · USMayo Clinic · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundToll-like receptors (TLRs) recognize known molecules from microbes and have an established role in tumorigenesis. Using a rat model of esophageal adenocarcinoma, and human clinical samples, we investigated genes central to TLR-mediated signal transduction and characterized the esophageal microbiome across the spectrum of esophageal adenocarcinoma carcinogenesis.

methodsWe surgically induced bile/acid reflux in rats and their esophagi were harvested at 40 weeks post-surgery. Tissue samples from the model were selected for gene expression profiling. Additionally, for rat and human samples microbiome analysis was performed using PCR-ESI-MS-TOF technology with validation by fluorescence in situ hybridization.

resultsGene expression results in the rat model indicated a significant upregulation of TLRs 1-3, 6, 7 and 9 in EAC compared to normal epithelium. PCR-ESI-MS-TOF analysis revealed a prevalence of Escherichia coli in Barrett's esophagus (60%) and esophageal adenocarcinoma (100%), which was validated by fluorescence in situ hybridization. In the human clinical samples, Streptococcus pneumonia was detected in high abundance in gastroesophageal reflux disease and Barrett's esophagus (50-70%) in comparison to tumor adjacent normal epithelium, dysplasia, and esophageal adenocarcinoma (20-30%). E. coli was detected in the Barrett's esophagus and esophageal adenocarcinoma groups but was absent in the tumor adjacent normal epithelium, dysplasia, and the gastroesophageal reflux disease groups.

conclusionsWe demonstrated an association between the TLR signaling pathway and E. coli hinting towards possible early molecular changes being mediated by microbes in the rat model of esophageal adenocarcinoma carcinogenesis. Studies on human clinical samples also corroborate results to some extent; however, a study with larger sample size is needed to further explore this association.

Indexed as

AdenocarcinomaAnimalsBarrett EsophagusCarcinogenesisDisease Models, AnimalEscherichia coliEsophageal NeoplasmsGene Expression Regulation, NeoplasticHumansIn Situ Hybridization, FluorescenceMicrobiotaRatsSignal TransductionStreptococcus pneumoniaeToll-Like ReceptorsToll-Like Receptors

Identifiers

PMID26841926
PMCPMC4739094
OpenAlexW2260354739

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.