ArticlePloS one2016
The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Fingers for signaling? A possible role of stromules in intracellular communication.Plant physiology · 2026Article
- Stromules: An Incident or Formalized Pathway for Molecules Transfer Between Organelles?International journal of molecular sciences · 2025Review
- Genome-wide identification of GA2ox genes family and analysis of PbrGA2ox1-mediated enhanced chlorophyll accumulation by promoting chloroplast development in pear.BMC plant biology · 2024Article
- Growth regulation by apyrases: Insights from altering their expression level in different organisms.Plant physiology · 2024Review
- Recent Advances Clarifying the Structure and Function of Plant Apyrases (Nucleoside Triphosphate Diphosphohydrolases).International journal of molecular sciences · 2021Review
- Red light imaging for programmed cell death visualization and quantification in plant-pathogen interactions.Molecular plant pathology · 2021Article
- Spatial chloroplast-to-nucleus signalling involving plastid-nuclear complexes and stromules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Ecotype-Specific Pathways of Reactive Oxygen Species Deactivation in Facultative MetallophyteAntioxidants (Basel, Switzerland) · 2020Article
- Ties that bind: the integration of plastid signalling pathways in plant cell metabolism.Essays in biochemistry · 2018Review
- Article
- A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication.Frontiers in cell and developmental biology · 2018Review
- Stromules: Probing Formation and Function.Plant physiology · 2018Review
- Visualizing Stromule Frequency with Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stromules are stroma-containing tubules that have been observed to emanate from the main plastidic body in vivo. These structures have been shown to require cytoskeletal components for movement. Though numerous studies have shown a close association with the endoplasmic reticulum, nucleus, mitochondria, and other plastids, the mechanism of formation and their overall function remain unknown. A limiting factor in studying these structures has been the lack of a reconstituted system for in vitro stromule formation. In this study, stromule formation was induced in vitro by adding a plant extract fraction that is greater than 100 kDa to a population of isolated chloroplasts. Kinetic measurements show that stromule formation occurs within ~10 seconds after the addition of the plant extract fraction. Heat inactivation and apyrase treatment reveal that the stromule stimulating compound found in the extract fraction is a protein or protein complex 100 kDa or greater. The formation of the stromules in vitro with isolated chloroplasts and a concentrated fraction of cell extract opens an avenue for the biochemical dissection of this process that has heretofore been studied only in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.