ArticleCellular microbiology2016
A MORN1-associated HAD phosphatase in the basal complex is essential for Toxoplasma gondii daughter budding.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Transmembrane proteins mediate basal complex assembly and individual daughter cell formation in malaria parasites.Science advances · 2026Article
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- Co-dependent formation of thebioRxiv : the preprint server for biology · 2024Article
- Immunization with Live-Attenuated RHΔPathogens (Basel, Switzerland) · 2024Article
- A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum.Nature communications · 2023Article
- Plasmodium schizogony, a chronology of the parasite's cell cycle in the blood stage.PLoS pathogens · 2023Review
- Identification of basal complex protein that is essential for maturation of transmission-stage malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.Nature communications · 2022Article
- The BCC7 Protein Contributes to theInternational journal of molecular sciences · 2022Article
- Epigenome-wide gene-age interaction study reveals reversed effects ofFrontiers in oncology · 2022Article
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- Apicoplast Dynamics DuringFrontiers in cellular and infection microbiology · 2022Review
- Identification of new components of the basal pole ofFrontiers in cellular and infection microbiology · 2022Article
- The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems.Frontiers in cellular and infection microbiology · 2021Review
- The Dynamic Roles of the Inner Membrane Complex in the Multiple Stages of the Malaria Parasite.Frontiers in cellular and infection microbiology · 2020Review
- The apical annuli of Toxoplasma gondii are composed of coiled-coil and signalling proteins embedded in the inner membrane complex sutures.Cellular microbiology · 2020Article
- An essential contractile ring protein controls cell division in Plasmodium falciparum.Nature communications · 2019Article
- Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.Frontiers in cellular and infection microbiology · 2019Review
- An evolutionarily conserved SSNA1/DIP13 homologue is a component of both basal and apical complexes of Toxoplasma gondii.Scientific reports · 2016Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Apicomplexan parasites replicate by several budding mechanisms with two well-characterized examples being Toxoplasma endodyogeny and Plasmodium schizogony. Completion of budding requires the tapering of the nascent daughter buds toward the basal end, driven by contraction of the basal complex. This contraction is not executed by any of the known cell division associated contractile mechanisms and in order to reveal new components of the unusual basal complex we performed a yeast two-hybrid screen with its major scaffolding protein, TgMORN1. Here we report on a conserved protein with a haloacid dehalogenase (HAD) phosphatase domain, hereafter named HAD2a, identified by yeast two-hybrid. HAD2a has demonstrated enzyme-activity in vitro, localizes to the nascent daughter buds, and co-localizes with MORN1 to the basal complex during its contraction. Conditional knockout of HAD2a in Toxoplasma interferes with basal complex assembly, which leads to incomplete cytokinesis and conjoined daughters that ultimately results in disrupted proliferation. In Plasmodium, we further confirmed localization of the HAD2a ortholog to the basal complex toward the end of schizogony. In conclusion, our work highlights an essential role for this HAD phosphatase across apicomplexan budding and suggests a regulatory mechanism of differential phosphorylation on the structure and/or contractile function of the basal complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.