ArticlePloS one2016
Co-Occurrence of Two Allelic Variants of CYP51 in Erysiphe necator and Their Correlation with Over-Expression for DMI Resistance.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Evaluation of Fungicide Resistance of the Brown Shot-hole Pathogens of Cherry Trees in Korea.The plant pathology journal · 2026Article
- From crops to clinic: the impact of dual azole use on antifungal resistance inFrontiers in microbiology · 2025Review
- Multiple routes to fungicide resistance: Interaction of Cyp51 gene sequences, copy number and expression.Molecular plant pathology · 2024Article
- Comprehensive analyses of the occurrence of a fungicide resistance marker and the genetic structure in Erysiphe necator populations.Scientific reports · 2023Article
- Article
- Identification of the Pathogen Causing Leaf Spot inPathogens (Basel, Switzerland) · 2022Article
- Multiple Mutations and Overexpression in theCurrent issues in molecular biology · 2022Article
- Identification ofFrontiers in plant science · 2022Article
- Molecular characterization and overexpression of the difenoconazole resistance gene CYP51 in Lasiodiplodia theobromae field isolates.Scientific reports · 2021Article
- Molecular Mechanisms Underlying Fungicide Resistance in Citrus Postharvest Green Mold.Journal of fungi (Basel, Switzerland) · 2021Review
- Molecular Characterization of Laboratory Mutants ofJournal of fungi (Basel, Switzerland) · 2021Article
- DMI-Fungicide Resistance inMicroorganisms · 2021Article
- Article
- Fungicide Resistance in Powdery Mildew Fungi.Microorganisms · 2020Review
- Pyridachlometyl has a novel anti-tubulin mode of action which could be useful in anti-resistance management.Pest management science · 2020Article
- A new mechanism for reduced sensitivity to demethylation-inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis.Molecular plant pathology · 2018Article
- Demethylase Inhibitor Fungicide Resistance in Pyrenophora teres f. sp. teres Associated with Target Site Modification and Inducible Overexpression of Cyp51.Frontiers in microbiology · 2016Article
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Demethylation inhibitors (DMIs) have been an important tool in the management of grapevine powdery mildew caused by Erysiphe necator. Long-term, intensive use of DMIs has resulted in reduced sensitivity in field populations. To further characterize DMI resistance and understand resistance mechanisms in this pathogen, we investigated the cyp51 sequence of 24 single-spored isolates from Virginia and surrounding states and analyzed gene expression in isolates representing a wide range of sensitivity. Two cyp51 alleles were found with respect to the 136th codon of the predicted EnCYP51 sequence: the wild-type (TAT) and the mutant (TTT), which results in the known Y136F amino acid change. Some isolates possessed both alleles, demonstrating gene duplication or increased gene copy number and possibly a requirement for at least one mutant copy of CYP51 for resistance. Cyp51 was over-expressed 1.4- to 19-fold in Y136F-mutant isolates. However, the Y136F mutation was absent in one isolate with moderate to high resistance factor. Two additional synonymous mutations were detected as well, one of which, A1119C was present only in isolates with high cyp51 expression. Overall, our results indicate that at least two mechanisms, cyp51 over-expression and the known target-site mutation in CYP51, contribute to resistance in E. necator, and may be working in conjunction with each other.
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