ArticleNeurophotonics2015
Serotype-dependent transduction efficiencies of recombinant adeno-associated viral vectors in monkey neocortex.
Article in Neurophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Optotagging in primate cortex uncovers excitatory and inhibitory neuron specializations in form vision.Nature communications · 2026Article
- Local stimulation of pyramidal neurons in deep cortical layers of anesthetized rats enhances cortical visual information processing.Scientific reports · 2024Article
- Development and characterization of a non-human primate model of disseminated synucleinopathy.Frontiers in neuroanatomy · 2024Article
- Direct Comparison of Epifluorescence and Immunostaining for Assessing Viral Mediated Gene Expression in the Primate Brain.Human gene therapy · 2023Article
- Multi-area recordings and optogenetics in the awake, behaving marmoset.Nature communications · 2023Article
- Optogenetics in primate cortical networks.Frontiers in neuroanatomy · 2023Article
- Applications of chemogenetics in non-human primates.Current opinion in pharmacology · 2022Review
- Chemogenetic Seizure Control with Clozapine and the Novel Ligand JHU37160 Outperforms the Effects of Levetiracetam in the Intrahippocampal Kainic Acid Mouse Model.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2022Article
- Specific gene expression in unmyelinated dorsal root ganglion neurons in nonhuman primates by intra-nerve injection of AAV 6 vector.Molecular therapy. Methods & clinical development · 2021Article
- Combining brain perturbation and neuroimaging in non-human primates.NeuroImage · 2021Review
- Cortical gamma-band resonance preferentially transmits coherent input.Cell reports · 2021Article
- Nonhuman Primate Optogenetics: Current Status and Future Prospects.Advances in experimental medicine and biology · 2021Review
- An Open Resource for Non-human Primate Optogenetics.Neuron · 2020Article
- Comparative analyses of transgene expression patterns after intra-striatal injections of rAAV2-retro in rats and rhesus monkeys: A light and electron microscopic study.The European journal of neuroscience · 2020Article
- Tissue-Specific Ferritin- and GFP-Based Genetic Vectors Visualize Neurons by MRI in the Intact and Post-Ischemic Rat Brain.International journal of molecular sciences · 2020Article
- Using rAAV2-retro in rhesus macaques: Promise and caveats for circuit manipulation.Journal of neuroscience methods · 2020Review
- Methods for mechanical delivery of viral vectors into rhesus monkey brain.Journal of neuroscience methods · 2020Article
- Mouse γ-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics.Gene therapy · 2020Article
- Optogenetic Stimulation of the Superior Colliculus Confers Retinal Neuroprotection in a Mouse Glaucoma Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral vector-mediated expression of genes (e.g., coding for opsins and designer receptors) has grown increasingly popular. Cell-type specific expression is achieved by altering viral vector tropism through crosspackaging or by cell-specific promoters driving gene expression. Detailed information about transduction properties of most recombinant adeno-associated viral vector (rAAV) serotypes in macaque cortex is gradually becoming available. Here, we compare transduction efficiencies and expression patterns of reporter genes in two macaque neocortical areas employing different rAAV serotypes and promoters. A short version of the calmodulin-kinase-II (CaMKIIα0.4) promoter resulted in reporter gene expression in cortical neurons for all tested rAAVs, albeit with different efficiencies for spread: rAAV2/5>>rAAV2/7>rAAV2/8>rAAV2/9>>rAAV2/1 and proportion of transduced cells: rAAV2/1>rAAV2/5>rAAV2/7=rAAV2/9>rAAV2/8. In contrast to rodent studies, the cytomegalovirus (CMV) promoter appeared least efficient in macaque cortex. The human synapsin-1 promoter preceded by the CMV enhancer (enhSyn1) produced homogeneous reporter gene expression across all layers, while two variants of the CaMKIIα promoter resulted in different laminar transduction patterns and cell specificities. Finally, differences in expression patterns were observed when the same viral vector was injected in two neocortical areas. Our results corroborate previous findings that reporter-gene expression patterns and efficiency of rAAV transduction depend on serotype, promoter, cortical layer, and area.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.