Evidence map›Paper›PMID 26839408›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Differential vesicular sorting of AMPA and GABAA receptors.

Yi Gu, Shu-Ling Chiu, Bian Liu, Pei-Hsun Wu, Michael Delannoy, Da-Ting Lin, Denis Wirtz, Richard L Huganir

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 79 citations in OpenAlex.

  1. GABASynapse (New York, N.Y.) · 2026
    Review
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  4. Synaptobrevin-2 disease variants reveal spatial constraints within the presynaptic active zone.Proceedings of the National Academy of Sciences of the United States of America · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yi GuDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
Shu-Ling ChiuDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
Bian LiuDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
Pei-Hsun WuDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218;
Michael DelannoyJohns Hopkins University School of Medicine Microscope Facility, Baltimore, MD 21205.
Da-Ting LinDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
Denis WirtzDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218;
Richard L HuganirDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205; rhuganir@jhmi.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

In vivo imaging of dorsal striatum function in freely behaving miceZIADA000603 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI LIN, DA-TING · 2015 to 2025
$22.3M
PDZ DOMAINS AND EXCITATORY SYNAPTIC FUNCTIONR01NS036715 · NINDS · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L · 1997 to 2020
$8.6M
Regulation of the NMDA Receptor Signaling ComplexR01MH064856 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L · 2002 to 2011
$3.6M
NIMH NIH HHS R01 MH064856NIMH NIH HHS R01 MH64856NINDS NIH HHS R01 NS036715
6 · The paper itself

Abstract

In mature neurons AMPA receptors cluster at excitatory synapses primarily on dendritic spines, whereas GABAA receptors cluster at inhibitory synapses mainly on the soma and dendritic shafts. The molecular mechanisms underlying the precise sorting of these receptors remain unclear. By directly studying the constitutive exocytic vesicles of AMPA and GABAA receptors in vitro and in vivo, we demonstrate that they are initially sorted into different vesicles in the Golgi apparatus and inserted into distinct domains of the plasma membrane. These insertions are dependent on distinct Rab GTPases and SNARE complexes. The insertion of AMPA receptors requires SNAP25-syntaxin1A/B-VAMP2 complexes, whereas insertion of GABAA receptors relies on SNAP23-syntaxin1A/B-VAMP2 complexes. These SNARE complexes affect surface targeting of AMPA or GABAA receptors and synaptic transmission. Our studies reveal vesicular sorting mechanisms controlling the constitutive exocytosis of AMPA and GABAA receptors, which are critical for the regulation of excitatory and inhibitory responses in neurons.

Indexed as

AnimalsCell MembraneCells, CulturedExocytosisGolgi ApparatusPyramidal CellsRatsReceptors, AMPAReceptors, GABA-ASyntaxin 1Vesicle-Associated Membrane Protein 2Receptors, AMPAReceptors, GABA-ASyntaxin 1Vamp2 protein, ratVesicle-Associated Membrane Protein 2AMPA receptorconstitutive exocytosisGABAA receptorSNARETIRFM

Identifiers

PMID26839408
PMCPMC4763724
OpenAlexW2254068781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.