ArticleDevelopment (Cambridge, England)2016
Mammary epithelial tubes elongate through MAPK-dependent coordination of cell migration.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 80 citations in OpenAlex.
- Microtubule dependent changes in cell polarity are required for mammary epithelial cell migration and branching morphogenesis.Biology open · 2025Article
- Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung.Developmental biology · 2025Article
- Sympathetic nerve signals: orchestrators of mammary development and stem cell vitality.Journal of molecular cell biology · 2024Article
- Using Microfluidics to Align Matrix Architecture and Generate Chemokine Gradients Promotes Directional Branching in a Model of Epithelial Morphogenesis.ACS biomaterials science & engineering · 2024Article
- Organoid Cultures for the Study of Mammary Biology and Breast Cancer: The Promise and Challenges.Cold Spring Harbor perspectives in medicine · 2024Review
- Fibroblast-induced mammary epithelial branching depends on fibroblast contractility.PLoS biology · 2024Article
- Article
- Spatially coordinated cell cycle activity and motility govern bifurcation of mammary branches.The Journal of cell biology · 2023Article
- Collective cell migration is spatiotemporally regulated during mammary epithelial bifurcation.Journal of cell science · 2023Article
- Assessment of microRNA profiles in small extracellular vesicles isolated from bovine colostrum with different immunoglobulin G concentrations.JDS communications · 2022Article
- Mechanical coupling of supracellular stress amplification and tissue fluidization during exit from quiescence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Dynamic miRNA Landscape Links Mammary Gland Development to the Regulation of Milk Protein Expression in Mice.Animals : an open access journal from MDPI · 2022Article
- Characterization of RNA Editome in the Mammary Gland of Yaks during the Lactation and Dry Periods.Animals : an open access journal from MDPI · 2022Article
- Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation.eLife · 2021Article
- Collective behaviours in organoids.Current opinion in cell biology · 2021Review
- BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models.Nature communications · 2021Article
- Local accumulation of extracellular matrix regulates global morphogenetic patterning in the developing mammary gland.Current biology : CB · 2021Article
- Ret signaling in ureteric bud epithelial cells controls cell movements, cell clustering and bud formation.Development (Cambridge, England) · 2021Article
- A Robust Mammary Organoid System to Model Lactation and Involution-like Processes.Bio-protocol · 2021Article
- The Cellular Organization of the Mammary Gland: Insights From Microscopy.Journal of mammary gland biology and neoplasia · 2021Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Mammary branching morphogenesis is regulated by receptor tyrosine kinases (RTKs). We sought to determine how these RTK signals alter proliferation and migration to accomplish tube elongation in mouse. Both behaviors occur but it has been difficult to determine their relative contribution to elongation in vivo, as mammary adipocytes scatter light and limit the depth of optical imaging. Accordingly, we utilized 3D culture to study elongation in an experimentally accessible setting. We first used antibodies to localize RTK signals and discovered that phosphorylated ERK1/2 (pERK) was spatially enriched in cells near the front of elongating ducts, whereas phosphorylated AKT was ubiquitous. We next observed a gradient of cell migration speeds from rear to front of elongating ducts, with the front characterized by both high pERK and the fastest cells. Furthermore, cells within elongating ducts oriented both their protrusions and their migration in the direction of tube elongation. By contrast, cells within the organoid body were isotropically protrusive. We next tested the requirement for proliferation and migration. Early inhibition of proliferation blocked the creation of migratory cells, whereas late inhibition of proliferation did not block continued duct elongation. By contrast, pharmacological inhibition of either MEK or Rac1 signaling acutely blocked both cell migration and duct elongation. Finally, conditional induction of MEK activity was sufficient to induce collective cell migration and ductal elongation. Our data suggest a model for ductal elongation in which RTK-dependent proliferation creates motile cells with high pERK, the collective migration of which acutely requires both MEK and Rac1 signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.