ArticleDevelopment (Cambridge, England)2016
Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- ER shaping proteins guide spindle elongation and division during rapid cleavage mitoses.bioRxiv : the preprint server for biology · 2026Article
- Drosophila embryo cellularization is tuned by the viscoelastic properties of membrane-cortex linkers.Biophysical journal · 2026Article
- MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos.The Journal of cell biology · 2026Article
- Article
- Centrosome-organized plasma membrane infoldings linked to growth of a cortical actin domain.The Journal of cell biology · 2024Article
- Basement membrane diversification relies on two competitive secretory routes defined by Rab10 and Rab8 and modulated by dystrophin and the exocyst complex.PLoS genetics · 2024Article
- Reshaping the Syncytial Drosophila Embryo with Cortical Actin Networks: Four Main Steps of Early Development.Results and problems in cell differentiation · 2024Review
- Dysregulation of the endoplasmic reticulum blocks recruitment of centrosome-associated proteins resulting in mitotic failure.Development (Cambridge, England) · 2023Article
- A Rab39-Klp98A-Rab35 endocytic recycling pathway is essential for rapid Golgi-dependent furrow ingression.Development (Cambridge, England) · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
- Actomyosin activity-dependent apical targeting of Rab11 vesicles reinforces apical constriction.The Journal of cell biology · 2022Article
- Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos.Journal of visualized experiments : JoVE · 2021Article
- Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo.eLife · 2021Article
- Transcytosis in the development and morphogenesis of epithelial tissues.The EMBO journal · 2021Review
- Article
- Rabs in Signaling and Embryonic Development.International journal of molecular sciences · 2020Review
- Key roles of Arf small G proteins and biosynthetic trafficking for animal development.Small GTPases · 2019Review
- Nuf and Rip11 requirement for polarity determinant recycling during Drosophila development.Small GTPases · 2018Article
- Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response.Cell reports · 2017Article
- Endocytic turnover of Rab8 controls cell polarization.Journal of cell science · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
One of the most fundamental changes in cell morphology is the ingression of a plasma membrane furrow. The Drosophila embryo undergoes several cycles of rapid furrow ingression during early development that culminate in the formation of an epithelial sheet. Previous studies have demonstrated the requirement for intracellular trafficking pathways in furrow ingression; however, the pathways that link compartmental behaviors with cortical furrow ingression events are unclear. Here, we show that Rab8 has striking dynamic behaviors in vivo. As furrows ingress, cytoplasmic Rab8 puncta are depleted and Rab8 accumulates at the plasma membrane in a location that coincides with known regions of directed membrane addition. We additionally use CRISPR/Cas9 technology to N-terminally tag Rab8, which is then used to address endogenous localization and function. Endogenous Rab8 displays partial coincidence with Rab11 and the Golgi, and this colocalization is enriched during the fast phase of cellularization. When Rab8 function is disrupted, furrow formation in the early embryo is completely abolished. We also demonstrate that Rab8 behaviors require the function of the exocyst complex subunit Sec5 as well as the recycling endosome protein Rab11. Active, GTP-locked Rab8 is primarily associated with dynamic membrane compartments and the plasma membrane, whereas GDP-locked Rab8 forms large cytoplasmic aggregates. These studies suggest a model in which active Rab8 populations direct furrow ingression by guiding the targeted delivery of cytoplasmic membrane stores to the cell surface through interactions with the exocyst tethering complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.