Evidence map›Paper›PMID 26838270›Full record

ArticleStem cells translational medicine2016

Comparative Analysis of Media and Supplements on Initiation and Expansion of Adipose-Derived Stem Cells.

Simone Riis, Frederik Mølgaard Nielsen, Cristian Pablo Pennisi, Vladimir Zachar, Trine Fink

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 2 pooled it
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 73 citations in OpenAlex.

  1. Pooled it
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  7. Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Simone RiisLaboratory for Stem Cell Research, Department for Health Science and Technology, Aalborg University, Aalborg, Denmark.
Frederik Mølgaard NielsenLaboratory for Stem Cell Research, Department for Health Science and Technology, Aalborg University, Aalborg, Denmark.
Cristian Pablo PennisiLaboratory for Stem Cell Research, Department for Health Science and Technology, Aalborg University, Aalborg, Denmark.
Vladimir ZacharLaboratory for Stem Cell Research, Department for Health Science and Technology, Aalborg University, Aalborg, Denmark.
Trine FinkLaboratory for Stem Cell Research, Department for Health Science and Technology, Aalborg University, Aalborg, Denmark trinef@hst.aau.dk.
Aalborg University · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ASCs) are being tested in clinical trials related to cell-based regenerative therapies. Although most of the current expansion protocols for ASCs use fetal calf serum (FCS), xenogeneic-free medium supplements are greatly desired. This study aims to compare the effect of FCS, human platelet lysate (hPL), and a fully defined medium on the initiation and maintenance of ASC cultures. ASCs obtained from five donors were cultured in five different media: StemPro, Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% hPL, or α-minimum essential medium (A-MEM) supplemented with 5% hPL, 10% hPL, or 10% FCS. The effect of media on proliferation, colony-forming units (CFUs), attachment, and morphology was assessed along with cell size, granularity, and immunophenotype. StemPro greatly compromised the initiation of ASC cultures, which could not survive more than a few passages. Cells cultured in A-MEM proliferated at a faster rate than in DMEM, and hPL significantly enhanced cell size, granularity, and proliferation compared with FCS. All media except StemPro supported CFUs equally well. Analysis of surface markers revealed higher levels of CD73 and CD105 in FCS-cultured ASCs, whereas increased levels of CD146 were found in hPL-cultured cells. Multiparametric flow cytometric analysis performed after seven passages revealed the existence of four distinct ASC subpopulations, all positive for CD73, CD90, and CD105, which mainly differed by their expression of CD146 and CD271. Analysis of the different subpopulations might represent an important biological measure when assessing different medium formulations for a particular clinical application.

Indexed as

AdipocytesAdipose TissueAnimalsBlood PlateletsCattleCell- and Tissue-Based TherapyCell DifferentiationCell ProliferationCulture Media, Serum-FreeFlow CytometryHumansRegenerative MedicineStem CellsCulture Media, Serum-FreeAdipose-derived stem cellsCell proliferationCell therapyCulture mediaPhenotypeXenogeneic free

Identifiers

PMID26838270
PMCPMC4807663
OpenAlexW2253228009

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.