ArticleG3 (Bethesda, Md.)2016
Genomic Access to Monarch Migration Using TALEN and CRISPR/Cas9-Mediated Targeted Mutagenesis.
Article in G3 (Bethesda, Md.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- To Migrate or not to Migrate? Exploring the Genomic Basis of Partial Migratory Behavior in Bats.Genome biology and evolution · 2025Article
- Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption.PloS one · 2025Article
- High-Throughput Screening of PAM-Flexible Cas9 Variants for Expanded Genome Editing in the Silkworm (Insects · 2024Article
- A perspective on neuroethology: what the past teaches us about the future of neuroethology.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Loss of functionaliScience · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Migration genetics take flight: genetic and genomic insights into monarch butterfly migration.Current opinion in insect science · 2023Review
- Insect magnetoreception: a Cry for mechanistic insights.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2023Review
- Chromosome-Level Genome Assembly ofInsects · 2023Article
- Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects.BMC biology · 2023Article
- Anthropogenic Zinc Exposure Increases Mortality and Antioxidant Gene Expression in Monarch Butterflies with Low Access to Dietary Macronutrients.Environmental toxicology and chemistry · 2022Article
- TRITHORAX-dependent arginine methylation of HSP68 mediates circadian repression by PERIOD in the monarch butterfly.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Role of Experiments in Monarch Butterfly Conservation: A Review of Recent Studies and Approaches.Annals of the Entomological Society of America · 2022Article
- Cryptochrome 1 mediates light-dependent inclination magnetosensing in monarch butterflies.Nature communications · 2021Article
- Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes.Scientific reports · 2021Article
- The Circadian Clock in Lepidoptera.Frontiers in physiology · 2021Review
- Article
- Monarch Butterfly Migration Moving into the Genetic Era.Trends in genetics : TIG · 2020Review
- How to turn an organism into a model organism in 10 'easy' steps.The Journal of experimental biology · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The eastern North American monarch butterfly, Danaus plexippus, is an emerging model system to study the neural, molecular, and genetic basis of animal long-distance migration and animal clockwork mechanisms. While genomic studies have provided new insight into migration-associated and circadian clock genes, the general lack of simple and versatile reverse-genetic methods has limited in vivo functional analysis of candidate genes in this species. Here, we report the establishment of highly efficient and heritable gene mutagenesis methods in the monarch butterfly using transcriptional activator-like effector nucleases (TALENs) and CRISPR-associated RNA-guided nuclease Cas9 (CRISPR/Cas9). Using two clock gene loci, cryptochrome 2 and clock (clk), as candidates, we show that both TALENs and CRISPR/Cas9 generate high-frequency nonhomologous end-joining (NHEJ)-mediated mutations at targeted sites (up to 100%), and that injecting fewer than 100 eggs is sufficient to recover mutant progeny and generate monarch knockout lines in about 3 months. Our study also genetically defines monarch CLK as an essential component of the transcriptional activation complex of the circadian clock. The methods presented should not only greatly accelerate functional analyses of many aspects of monarch biology, but are also anticipated to facilitate the development of these tools in other nontraditional insect species as well as the development of homology-directed knock-ins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.