ArticleScientific reports2016
An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Engineering resistance genes against tomato brown rugose fruit virus.Science China. Life sciences · 2025Article
- Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.BMC plant biology · 2025Article
- Knockout of SlDCL2b attenuates the resistance of tomato to potato spindle tuber viroid infection.Molecular plant pathology · 2024Article
- Small RNAs: Efficient and miraculous effectors that play key roles in plant-microbe interactions.Molecular plant pathology · 2023Review
- iTRAQ based protein profile analysis revealed key proteins involved in regulation of drought-tolerance during seed germination in Adzuki bean.Scientific reports · 2021Article
- Suppression of nbe-miR1919c-5p Expression inViruses · 2020Article
- Plant NLR immune receptor Tm-22 activation requires NB-ARC domain-mediated self-association of CC domain.PLoS pathogens · 2020Article
- A Versatile Plant Rhabdovirus-Based Vector for Gene Silencing, miRNA Expression and Depletion, and Antibody Production.Frontiers in plant science · 2020Article
- Analyses of MiRNA Functions in Maize Using a Newly Developed ZMBJ-CMV-2bFrontiers in plant science · 2019Article
- Tomato lncRNA23468 functions as a competing endogenous RNA to modulateHorticulture research · 2019Article
- Retardation of the Calvin Cycle Contributes to the Reduced COFrontiers in microbiology · 2019Article
- Functional genomic approaches to improve crop plant heat stress tolerance.F1000Research · 2019Review
- Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation.Molecular plant pathology · 2018Article
- MicroRNAPlant direct · 2018Article
- Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase.PLoS pathogens · 2018Article
- Plant Responses to Pathogen Attack: Small RNAs in Focus.International journal of molecular sciences · 2018Review
- Function identification of miR482b, a negative regulator during tomato resistance toHorticulture research · 2018Article
- Hsp90 Interacts With Tm-2Frontiers in plant science · 2018Article
- A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants.Plant physiology · 2017Article
- Antiviral Resistance Protein Tm-2Plant physiology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant microRNAs (miRNAs) play pivotal roles in many biological processes. Although many miRNAs have been identified in various plant species, the functions of these miRNAs remain largely unknown due to the shortage of effective genetic tools to block their functional activity. Recently, miRNA target mimic (TM) technologies have been applied to perturb the activity of specific endogenous miRNA or miRNA families. We previously reported that Tobacco rattle virus (TRV)-based TM expression can successfully mediate virus-based miRNA silencing/suppression (VbMS) in plants. In this study, we show the Potato virus X (PVX)-based TM expression causes strong miRNA silencing in Nicotiana benthamiana. The PVX-based expression of short tandem target mimic (STTMs) against miR165/166 and 159 caused the corresponding phenotype in all infected plants. Thus, a PVX-based VbMS is a powerful method to study miRNA function and may be useful for high-throughput investigation of miRNA function in N. benthamiana.
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