ArticleNucleic acids research2016
Comparative transcriptomics elucidates adaptive phenol tolerance and utilization in lipid-accumulating Rhodococcus opacus PD630.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
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- Evolution of pollutant biodegradation.Applied microbiology and biotechnology · 2025Review
- Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators.Biotechnology for biofuels and bioproducts · 2024Article
- Phenolic compounds induce ferroptosis-like death by promoting hydroxyl radical generation in the Fenton reaction.Communications biology · 2024Article
- Unveiling superior phenol detoxification and degradation ability inFrontiers in microbiology · 2024Article
- Comparative Genomic and Transcriptomic Analysis of Phenol Degradation and Tolerance inInternational journal of molecular sciences · 2023Article
- Transcriptomic analysis approach towards an improved tolerance of Escherichia coli to gallic acid stress.Archives of microbiology · 2023Article
- A High-Quality Genome-Scale Model forACS synthetic biology · 2023Article
- Report of genome sequence of3 Biotech · 2023Article
- Deciphering the transcriptional regulation of the catabolism of lignin-derived aromatics in Rhodococcus opacus PD630.Communications biology · 2022Article
- Comparative genomics reveals response of Rhodococcus pyridinivorans B403 to phenol after evolution.Applied microbiology and biotechnology · 2022Article
- Microbe-assisted phytoremediation of environmental pollutants and energy recycling in sustainable agriculture.Archives of microbiology · 2021Review
- Systems biology and metabolic engineering of Rhodococcus for bioconversion and biosynthesis processes.Folia microbiologica · 2021Review
- Valorization of lignin components into gallate by integrated biological hydroxylation, O-demethylation, and aryl side-chain oxidation.Science advances · 2021Article
- A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing ofSynthetic and systems biotechnology · 2021Article
- The Complete Genome Sequence and Structure of the OleaginousFrontiers in bioengineering and biotechnology · 2021Article
- Biotechnology of Rhodococcus for the production of valuable compounds.Applied microbiology and biotechnology · 2020Review
- Time-course transcriptome analysis reveals the mechanisms of Burkholderia sp. adaptation to high phenol concentrations.Applied microbiology and biotechnology · 2020Article
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms.Journal of microbiology and biotechnology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignin-derived (e.g. phenolic) compounds can compromise the bioconversion of lignocellulosic biomass to fuels and chemicals due to their toxicity and recalcitrance. The lipid-accumulating bacterium Rhodococcus opacus PD630 has recently emerged as a promising microbial host for lignocellulose conversion to value-added products due to its natural ability to tolerate and utilize phenolics. To gain a better understanding of its phenolic tolerance and utilization mechanisms, we adaptively evolved R. opacus over 40 passages using phenol as its sole carbon source (up to 373% growth improvement over wild-type), and extensively characterized two strains from passages 33 and 40. The two adapted strains showed higher phenol consumption rates (∼20 mg/l/h) and ∼2-fold higher lipid production from phenol than the wild-type strain. Whole-genome sequencing and comparative transcriptomics identified highly-upregulated degradation pathways and putative transporters for phenol in both adapted strains, highlighting the important linkage between mechanisms of regulated phenol uptake, utilization, and evolved tolerance. Our study shows that the R. opacus mutants are likely to use their transporters to import phenol rather than export them, suggesting a new aromatic tolerance mechanism. The identified tolerance genes and pathways are promising candidates for future metabolic engineering in R. opacus for improved lignin conversion to lipid-based products.
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